Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Brockman, H.L.; Wood, W.A.
    D-lactate dehydrogenase of Peptostreptococcus elsdenii (1975), Methods Enzymol., 41B, 309-312.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.2.4 o-phenanthroline very strong, reactivation with Zn2+ and Co2+ Megasphaera elsdenii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.4 0.013
-
 D-lactate with ferricyanide Megasphaera elsdenii
1.1.2.4 0.23
-
 ferricyanide
-
 Megasphaera elsdenii
1.1.2.4 26
-
 D-lactate with complete electron transport system Megasphaera elsdenii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.4 Co2+ restores activity after inhibition with o-phenanthroline Megasphaera elsdenii
1.1.2.4 ferricyanide can substitute for cytochrome c Megasphaera elsdenii
1.1.2.4 Zn2+ restores activity after inhibition with o-phenanthroline Megasphaera elsdenii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.4 125000
-
-
 Megasphaera elsdenii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.4 (R)-lactate + 2 ferricytochrome c Megasphaera elsdenii
-
 pyruvate + ferrocytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.4 Megasphaera elsdenii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.4
-
 Megasphaera elsdenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.4 (R)-lactate + 2 ferricytochrome c
-
 Megasphaera elsdenii pyruvate + ferrocytochrome c
-
 ?
1.1.2.4 (R)-lactate + ferricytochrome c
-
 Megasphaera elsdenii pyruvate + ferrocytochrome c
-
 ?
1.1.2.4 D-2-hydroxybutyrate + cytochrome c
-
 Megasphaera elsdenii 2-oxobutanoate + ferrocytochrome c
-
 ?
1.1.2.4 D-lactate + ferricyanide
-
 Megasphaera elsdenii pyruvate + ferrocyanide + H+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.4 6 8
-
 Megasphaera elsdenii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.4 cytochrome c
-
 Megasphaera elsdenii
1.1.2.4 FAD
-
 Megasphaera elsdenii
1.1.2.4 additional information no activity with NAD+ or NADP+ Megasphaera elsdenii