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Literature summary extracted from

  • Olson, S.T.; Massey, V.
    Purification and properties of the flavoenzyme D-lactate dehydrogenase from Megasphaera elsdenii (1979), Biochemistry, 18, 4714-4723.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.2.4 dithiothreitol and bovine serum albumin required for stability Megasphaera elsdenii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.2.4 EDTA
-
 Megasphaera elsdenii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.4 0.0026
-
 D-lactate
-
 Megasphaera elsdenii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.4 Cu2+ rapid inactivation, can be restored by addition of dithiothreitol Megasphaera elsdenii
1.1.2.4 ferricyanide can substitute for cytochrome c Megasphaera elsdenii
1.1.2.4 Zn2+ required for activity Megasphaera elsdenii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.4 55000
-
 2 * 55000, sedimentation equilibrium centrifugation and SDS-PAGE Megasphaera elsdenii
1.1.2.4 105000
-
 sedimentation equilibrium centrifugation Megasphaera elsdenii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.4 (R)-lactate + 2 ferricytochrome c Megasphaera elsdenii
-
 pyruvate + ferrocytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.4 Megasphaera elsdenii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.4
-
 Megasphaera elsdenii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.4 20.3
-
 purified enzyme Megasphaera elsdenii

Storage Stability

EC Number Storage Stability Organism
1.1.2.4 4°C, in presence of dithiothreitol, stable Megasphaera elsdenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.4 (R)-lactate + 2 ferricytochrome c
-
 Megasphaera elsdenii pyruvate + ferrocytochrome c
-
 ?
1.1.2.4 (R)-lactate + ferricytochrome c
-
 Megasphaera elsdenii pyruvate + ferrocytochrome c
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.2.4 dimer 2 * 55000, sedimentation equilibrium centrifugation and SDS-PAGE Megasphaera elsdenii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.4 FAD 1 FAD per subunit Megasphaera elsdenii