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Literature summary extracted from
- Inducible or constitutive polyethylene glycol dehydrogenase involved in the aerobic metabolism of polyethylene glycol (1989), J. Ferment. Bioeng., 67, 300-302.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.20 | ethylamine | 30% activation, Pseudomonas sp. strain No. 43 | Pseudomonas sp. |  |
| 1.1.99.20 | methylamine | 30% activation, Pseudomonas sp. strain No. 43 | Pseudomonas sp. | |
| 1.1.99.20 | phenazine methosulfate | activation, accelerates dichlorophenolindophenol reduction | Pseudomonas sp. | |
| 1.1.99.20 | phenazine methosulfate | activation, accelerates dichlorophenolindophenol reduction | Flavobacterium sp. | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.20 | 1,4-benzoquinone | at 0.02-0.05 mM, strong inhibitor; competitive to 2,6-dichlorophenolindophenol | Flavobacterium sp. | |
| 1.1.99.20 | 1,4-benzoquinone | at 0.02-0.05 mM, strong inhibitor; competitive to 2,6-dichlorophenolindophenol | Pseudomonas sp. | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.99.20 | membrane | - |
Pseudomonas sp. | 16020 | - |
| 1.1.99.20 | membrane | - |
Flavobacterium sp. | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.20 | Ca2+ | 30% activation, Pseudomonas sp. strain No. 101 | Pseudomonas sp. | |
| 1.1.99.20 | additional information | no metal ion requirement | Pseudomonas sp. | |
| 1.1.99.20 | additional information | no metal ion requirement | Flavobacterium sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.20 | Flavobacterium sp. | - |
strains No.202 to 205 | - |
| 1.1.99.20 | Flavobacterium sp. | - |
synergistic mixed culture with Pseudomonas sp. | - |
| 1.1.99.20 | Pseudomonas sp. | - |
strains No.42, 43, 101, 103 | - |
| 1.1.99.20 | Pseudomonas sp. | - |
synergistic mixed culture with Flavobacterium sp. | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.20 | 0.028 | - |
strain No. 43 | Pseudomonas sp. |
| 1.1.99.20 | 0.035 | - |
strain No. 203, crude extract | Flavobacterium sp. |
| 1.1.99.20 | 0.164 | - |
strain No. 103 | Pseudomonas sp. |
| 1.1.99.20 | 1.2 | - |
mixed culture | Pseudomonas sp. |
| 1.1.99.20 | 1.2 | - |
mixed culture | Flavobacterium sp. |
| 1.1.99.20 | 2.68 | - |
strain No. 203, purified enzyme | Flavobacterium sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.20 | PEG-1000 + acceptor | - |
Pseudomonas sp. | ? | - |
? | |
| 1.1.99.20 | PEG-1000 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | PEG-200 + acceptor | - |
Pseudomonas sp. | ? | - |
? | |
| 1.1.99.20 | PEG-200 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | PEG-20000 + acceptor | - |
Pseudomonas sp. | ? | - |
? | |
| 1.1.99.20 | PEG-20000 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | PEG-300 + acceptor | - |
Pseudomonas sp. | ? | - |
? | |
| 1.1.99.20 | PEG-300 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | PEG-400 + acceptor | - |
Pseudomonas sp. | ? | - |
? | |
| 1.1.99.20 | PEG-400 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | PEG-4000 + acceptor | - |
Pseudomonas sp. | ? | - |
? | |
| 1.1.99.20 | PEG-4000 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | PEG-6000 + acceptor | - |
Pseudomonas sp. | ? | - |
? | |
| 1.1.99.20 | PEG-6000 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | polyethylene glycol + acceptor | constitutive quinoprotein enzyme, enzyme of Flavobacterium sp. No.203 inductive | Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | polyethylene glycol + acceptor | first and main step in aerobic PEG-metabolism | Pseudomonas sp. | ? | - |
? | |
| 1.1.99.20 | polyethylene glycol + acceptor | first and main step in aerobic PEG-metabolism | Flavobacterium sp. | ? | - |
? | |
| 1.1.99.20 | primary alcohol + acceptor | 2,6-dichlorophenolindophenol is the acceptor in vitro | Pseudomonas sp. | aldehyde + reduced acceptor | - |
? | |
| 1.1.99.20 | primary alcohol + acceptor | 2,6-dichlorophenolindophenol is the acceptor in vitro | Flavobacterium sp. | aldehyde + reduced acceptor | - |
? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.20 | 2,6-dichlorophenolindophenol | electron acceptor | Pseudomonas sp. | |
| 1.1.99.20 | 2,6-dichlorophenolindophenol | electron acceptor | Flavobacterium sp. | |
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