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Literature summary extracted from
- Gentisate 1,2-dioxygenase from pseudomonas. Purification, characterization, and comparison of the enzymes from Pseudomonas testosteroni and Pseudomonas acidovorans (1990), J. Biol. Chem., 265, 6301-6311.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.4 | ascorbate | - |
Comamonas testosteroni |  |
| 1.13.11.4 | ascorbate | - |
Delftia acidovorans | |
| 1.13.11.4 | dithiothreitol | - |
Comamonas testosteroni | |
| 1.13.11.4 | dithiothreitol | - |
Delftia acidovorans | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.13.11.4 | cysteine stabilizes | Comamonas testosteroni |
| 1.13.11.4 | cysteine stabilizes | Delftia acidovorans |
| 1.13.11.4 | Fe2+ stabilizes | Comamonas testosteroni |
| 1.13.11.4 | Fe2+ stabilizes | Delftia acidovorans |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.4 | 0.015 | - |
4-Fluorogentisate | - |
Delftia acidovorans | |
| 1.13.11.4 | 0.018 | - |
3-Bromogentisate | - |
Delftia acidovorans | |
| 1.13.11.4 | 0.025 | - |
4-Chlorogentisate | - |
Delftia acidovorans | |
| 1.13.11.4 | 0.027 | - |
3-Methylgentisate | - |
Delftia acidovorans | |
| 1.13.11.4 | 0.035 | - |
3-Fluorogentisate | - |
Delftia acidovorans | |
| 1.13.11.4 | 0.044 | - |
4-Fluorogentisate | - |
Comamonas testosteroni | |
| 1.13.11.4 | 0.045 | - |
1,4-dihydroxy-2-naphthoate | - |
Delftia acidovorans | |
| 1.13.11.4 | 0.048 | - |
3-Isopropylgentisate | - |
Delftia acidovorans | |
| 1.13.11.4 | 0.05 | - |
3-Fluorogentisate | - |
Comamonas testosteroni | |
| 1.13.11.4 | 0.057 | - |
4-Methylgentisate | - |
Delftia acidovorans | |
| 1.13.11.4 | 0.074 | - |
gentisate | - |
Delftia acidovorans | |
| 1.13.11.4 | 0.085 | - |
gentisate | - |
Comamonas testosteroni | |
| 1.13.11.4 | 0.096 | - |
O2 | - |
Comamonas testosteroni | |
| 1.13.11.4 | 0.14 | - |
3,4-Dimethylgentisate | - |
Delftia acidovorans | |
| 1.13.11.4 | 0.24 | - |
4-Chlorogentisate | - |
Comamonas testosteroni | |
| 1.13.11.4 | 0.29 | - |
1,4-dihydroxy-2-naphthoate | - |
Comamonas testosteroni | |
| 1.13.11.4 | 0.33 | - |
3-Bromogentisate | - |
Comamonas testosteroni | |
| 1.13.11.4 | 0.45 | - |
4-O-Methylgentisate | - |
Comamonas testosteroni | |
| 1.13.11.4 | 0.52 | - |
4-Methylgentisate | - |
Comamonas testosteroni | |
| 1.13.11.4 | 1.18 | - |
3-O-Methylgentisate | - |
Comamonas testosteroni | |
| 1.13.11.4 | 1.2 | - |
4-Isopropylgentisate | - |
Comamonas testosteroni | |
| 1.13.11.4 | 1.23 | - |
4-Isopropylgentisate | - |
Comamonas testosteroni | |
| 1.13.11.4 | 1.75 | - |
3-Methylgentisate | - |
Comamonas testosteroni | |
| 1.13.11.4 | 2.75 | - |
3-Isopropylgentisate | - |
Comamonas testosteroni | |
| 1.13.11.4 | 3.65 | - |
3-Ethylgentisate | - |
Comamonas testosteroni | |
| 1.13.11.4 | 7.7 | - |
3,4-Dimethylgentisate | - |
Comamonas testosteroni | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.4 | Iron | 1.9-3.9 mol Fe2+ per subunit | Comamonas testosteroni | |
| 1.13.11.4 | Iron | 1.3-3.2 mol Fe2+ per subunit | Delftia acidovorans | |
| 1.13.11.4 | Iron | (alphaFe)4 structure | Comamonas testosteroni | |
| 1.13.11.4 | Iron | (alphaFe)4 structure | Delftia acidovorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.4 | 40800 | - |
4 * 40800, SDS-PAGE | Comamonas testosteroni |
| 1.13.11.4 | 158000 | - |
gel filtration | Comamonas testosteroni |
| 1.13.11.4 | 164000 | - |
gel filtration | Delftia acidovorans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.4 | Comamonas testosteroni | - |
- |
- |
| 1.13.11.4 | Delftia acidovorans | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.13.11.4 | exposure of enzyme to oxidants, e.g. H2O2 or K3Fe(CN)6, at 1 mM concentrations results in complete loss of activity, even under anaerobic conditions | Comamonas testosteroni |
| 1.13.11.4 | exposure of enzyme to oxidants, e.g. H2O2 or K3Fe(CN)6, at 1 mM concentrations results in complete loss of activity, even under anaerobic conditions | Delftia acidovorans |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.4 | - |
Comamonas testosteroni |
| 1.13.11.4 | - |
Delftia acidovorans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.4 | 484 | - |
- |
Delftia acidovorans |
| 1.13.11.4 | 498 | - |
- |
Comamonas testosteroni |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.13.11.4 | -80°C, storage following rapid freezing in liquid N2, protein concentration above 10 mg/ml, about 15% loss of activity after 1 year | Comamonas testosteroni |
| 1.13.11.4 | -80°C, storage following rapid freezing in liquid N2, protein concentration above 10 mg/ml, about 15% loss of activity after 1 year | Delftia acidovorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.4 | 1,4-dihydroxy-2-naphthoate + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| 1.13.11.4 | 1,4-dihydroxy-2-naphthoate + O2 | - |
Delftia acidovorans | ? | - |
? | |
| 1.13.11.4 | 2,5-dihydroxybenzoate + O2 | - |
Comamonas testosteroni | maleylpyruvate | - |
? | |
| 1.13.11.4 | 2,5-dihydroxybenzoate + O2 | - |
Delftia acidovorans | maleylpyruvate | - |
? | |
| 1.13.11.4 | 3,4-dimethylgentisate + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| 1.13.11.4 | 3,4-dimethylgentisate + O2 | - |
Delftia acidovorans | ? | - |
? | |
| 1.13.11.4 | 3-bromogentisate + O2 | - |
Comamonas testosteroni | 3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 3-bromogentisate + O2 | - |
Delftia acidovorans | 3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 3-ethylgentisate + O2 | - |
Comamonas testosteroni | 3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 3-ethylgentisate + O2 | - |
Delftia acidovorans | 3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 3-fluorogentisate + O2 | - |
Comamonas testosteroni | 3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 3-fluorogentisate + O2 | - |
Delftia acidovorans | 3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 3-hydroxybenzoate + O2 | minor pathway | Comamonas testosteroni | 3,4-dihydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 3-hydroxybenzoate + O2 | main pathway | Delftia acidovorans | 3,4-dihydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 3-isopropylgentisate + O2 | - |
Comamonas testosteroni | 4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 3-isopropylgentisate + O2 | - |
Delftia acidovorans | 4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 3-methylgentisate + O2 | - |
Comamonas testosteroni | 4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 3-methylgentisate + O2 | - |
Delftia acidovorans | 4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 3-O-methylgentisate + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| 1.13.11.4 | 3-O-methylgentisate + O2 | - |
Delftia acidovorans | ? | - |
? | |
| 1.13.11.4 | 4-chlorogentisate + O2 | - |
Comamonas testosteroni | 2-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 4-chlorogentisate + O2 | - |
Delftia acidovorans | 2-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 4-fluorogentisate + O2 | - |
Comamonas testosteroni | 2-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 4-fluorogentisate + O2 | - |
Delftia acidovorans | 2-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 4-isopropylgentisate + O2 | - |
Comamonas testosteroni | 4-hydroxy-2-isopropyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 4-isopropylgentisate + O2 | - |
Delftia acidovorans | 4-hydroxy-2-isopropyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 4-methylgentisate + O2 | - |
Comamonas testosteroni | 4-hydroxy-2-methyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 4-methylgentisate + O2 | - |
Delftia acidovorans | 4-hydroxy-2-methyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
| 1.13.11.4 | 4-O-methylgentisate + O2 | - |
Comamonas testosteroni | ? | - |
? | |
| 1.13.11.4 | 4-O-methylgentisate + O2 | - |
Delftia acidovorans | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.4 | tetramer | 4 * 37200-39800, SDS-PAGE | Delftia acidovorans |
| 1.13.11.4 | tetramer | 4 * 40800, SDS-PAGE | Comamonas testosteroni |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.4 | 322 | 328 | gentisate | - |
Delftia acidovorans | |
| 1.13.11.4 | 367 | 642 | gentisate | - |
Comamonas testosteroni | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.4 | 7 | 9 | - |
Comamonas testosteroni |
| 1.13.11.4 | 7 | 9 | - |
Delftia acidovorans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.4 | 6 | 8 | unstable below and above | Comamonas testosteroni |
| 1.13.11.4 | 6 | 8 | unstable below and above | Delftia acidovorans |
| 1.13.11.4 | 7.4 | - |
highest stability | Comamonas testosteroni |
| 1.13.11.4 | 7.4 | - |
highest stability | Delftia acidovorans |
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