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Literature summary extracted from

  • Suarez, M.; Ferrer, E.; Martin, M.
    Purification and biochemical characterization of gentisate 1,2-dioxygenase from Klebsiella pneumoniae M5a1 (1996), FEMS Microbiol. Lett., 143, 89-95.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.13.11.4 glycerol, beta-mercaptoethanol stabilize Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.4 Cu2+ 1 mM, complete inactivation Klebsiella pneumoniae
1.13.11.4 EDTA 100 mM, 85% inactivation Klebsiella pneumoniae
1.13.11.4 Fe3+ 2 mM, complete inactivation Klebsiella pneumoniae
1.13.11.4 H2O2 3 mM, 90% inactivation Klebsiella pneumoniae
1.13.11.4 Mn2+ 10 mM, complete inactivation Klebsiella pneumoniae
1.13.11.4 Zn2+ 10 mM, complete inactivation Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.4 0.052
-
 gentisate
-
 Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.4 Iron Fe2+, 0.9-1 atom per subunit Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.4 38000
-
 4 * 38000, amino acid composition Klebsiella pneumoniae
1.13.11.4 159000
-
 gel filtration Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.4 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.4
-
 Klebsiella pneumoniae

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.13.11.4 H2O2-inactivated enzyme is restored by hydroxylamine or ferrous iron Klebsiella pneumoniae

Storage Stability

EC Number Storage Stability Organism
1.13.11.4 -20°C, addition of glycerol and beta-mercaptoethanol, 96 h, 25% of activity remains Klebsiella pneumoniae
1.13.11.4 4°C, 48 h, complete loss of activity Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.4 2,5-dihydroxybenzoate + O2
-
 Klebsiella pneumoniae maleylpyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.4 tetramer 4 * 38000, amino acid composition Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.4 30
-
-
 Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.4 8 9
-
 Klebsiella pneumoniae