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Literature summary extracted from

  • Kamin, H.; White-Stevens, R.H.; Presswood, R.P.
    Salicylate hydroxylase (1978), Methods Enzymol., 53, 527-543.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.1 Cl-
-
 Pseudomonas sp.
1.14.13.1 F-
-
 Pseudomonas sp.
1.14.13.1 I-
-
 Pseudomonas sp.
1.14.13.1 SCN-
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.1 0.0027
-
 salicylate cosubstrate NADH Pseudomonas sp.
1.14.13.1 0.011
-
 2,6-dihydroxybenzoate cosubstrate NADH Pseudomonas sp.
1.14.13.1 0.015
-
 p-Aminosalicylate cosubstrate NADH Pseudomonas sp.
1.14.13.1 0.017
-
 NADH cosubstrate salicylate Pseudomonas sp.
1.14.13.1 0.028
-
 2,3-Dihydroxybenzoate cosubstrate NADH Pseudomonas sp.
1.14.13.1 0.034
-
 o-Nitrophenol cosubstrate NADH Pseudomonas sp.
1.14.13.1 0.044
-
 NADH cosubstrate p-aminosalicylate Pseudomonas sp.
1.14.13.1 0.065
-
 2,5-Dihydroxybenzoate cosubstrate NADH Pseudomonas sp.
1.14.13.1 0.091
-
 NADH cosubstrate 2,3-dihydroxybenzoate Pseudomonas sp.
1.14.13.1 0.1
-
 NADPH cosubstrate salicylate Pseudomonas sp.
1.14.13.1 0.14
-
 NADH cosubstrate 2,4-dihydroxybenzoate Pseudomonas sp.
1.14.13.1 0.143
-
 2,4-dihydroxybenzoat cosubstrate NADH Pseudomonas sp.
1.14.13.1 0.23
-
 NADH cosubstrate 2,5-dihydroxybenzoate or 2,6-dihydroxybenzoate Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.1 43000 48700 gel filtration, sedimentation equilibrium, calculation of MW per flavin Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.1 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.1
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.1 10.57
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.1 2,3-dihydroxybenzoate + NADH + O2
-
 Pseudomonas sp. pyrogallol + CO2 + H2O + NAD+
-
 ?
1.14.13.1 2,4-dihydroxybenzoate + NADPH + H+ + O2
-
 Pseudomonas sp. benzene-1,2,4-triol + NAD+ + H2O + CO2
-
 r
1.14.13.1 2,5-dihydroxybenzoate + NADH + O2
-
 Pseudomonas sp. benzene-1,2,5-triol + CO2 + H2O + NAD+
-
 ?
1.14.13.1 2,6-dihydroxybenzoate + NADH + O2
-
 Pseudomonas sp. pyrogallol + CO2 + H2O + NAD+
-
 ?
1.14.13.1 m-hydroxybenzoate + NADH + O2 6% of the reaction with salicylate Pseudomonas sp. 1,3-dihydroxybenzene + CO2 + NAD+ + H2O
-
 ?
1.14.13.1 additional information mechanism Pseudomonas sp. ?
-
 ?
1.14.13.1 o-nitrophenol + NADH + O2
-
 Pseudomonas sp. catechol + nitrite + NAD+
-
 ?
1.14.13.1 p-aminosalicylate + NADH + O2
-
 Pseudomonas sp. 1,2-dihydroxy-4-aminobenzene + NAD+ + CO2 + H2O
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2
-
 Pseudomonas sp. catechol + NAD+ + H2O + CO2
-
 ?
1.14.13.1 salicylate + O2 + NADPH + 2 H+
-
 Pseudomonas sp. catechol + CO2 + H2O + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.1 dimer 2 * 43000-48700, sedimentation equilibrium Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.1 25 27 assay at Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.1 FAD flavoprotein Pseudomonas sp.
1.14.13.1 FAD 2 FAD per enzyme molecule, 2 subunits, total MW 91000 Pseudomonas sp.
1.14.13.1 NADH
-
 Pseudomonas sp.
1.14.13.1 NADPH
-
 Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.13.1 60
-
 Cl-
-
 Pseudomonas sp.