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Literature summary extracted from

  • Ullah, A.H.J.; Murray, R.I.; Bhattacharyya, P.K.; Wagner, G.C.; Gunsalus, I.C.
    Protein components of a cytochrome P-450 linalool 8-methyl hydroxylase (1990), J. Biol. Chem., 265, 1345-1351.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.84 CO complete inhibition; complete inhibition of the oxidation reactions Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.84 cytoplasm
-
 Pseudomonas putida 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.84 Fe2+ requirement of two enzyme components, iron-heme protein and iron-sulfur protein Pseudomonas putida
1.14.14.84 Fe2+ heme-containing cytochrome P450 enzyme Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.84 additional information
-
 multi-component enzyme consisting of LIN-reductase, Fe2-S2-redoxin and cytochrome LIN-P450. MW of the reductase: 43700 Da, analytical data from amino acid composition and prosthetic group quantification, 45000 Da, gel filtration. MW of Fe2-S2-redoxin: 11000 Da, gel filtration, 12800 Da, analytical data from amino acid composition and prosthetic group quantification. MW of cytochrome LIN-P450: 44800 Da, analytical data from amino acid composition and prosthetic group quantification, 45000 Da, gel filtration Pseudomonas putida
1.14.14.84 43700
-
 1 * 44000, SDS-PAGE, 1 * 43700, about, sequence calculation Pseudomonas putida
1.14.14.84 44000
-
 1 * 44000, SDS-PAGE, 1 * 43700, about, sequence calculation Pseudomonas putida
1.14.14.84 45000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.84 3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2 Pseudomonas putida linalool, initial reaction in linalool catabolism (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.84 3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2 Pseudomonas putida PpG777 linalool, initial reaction in linalool catabolism (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Pseudomonas putida
-
 (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
 ?
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Pseudomonas putida PpG777
-
 (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.84 Pseudomonas putida
-
-
-
1.14.14.84 Pseudomonas putida
-
 i.e. Pseudomonas incognita
-
1.14.14.84 Pseudomonas putida PpG777
-
 i.e. Pseudomonas incognita
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.84 native enzyme by anion exchange chromatography, ammonium sulfate fractionation, a second step of anion exchange chromatography, affinity chromatography, a thrid step of anion exchange chromatography, and two further different steps of affinity chromatography to over 90% hommogeneity Pseudomonas putida
1.14.14.84 purification of 3 enzyme components: iron-sulphur, FAD and P-450 Pseudomonas putida

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 = (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O mixed function monooxygenase consisting of LIN-reductase, Fe2S2-redoxin and cytochrome LIN P-450 Pseudomonas putida
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.84 additional information strain PpG777 utilizes linalool as sole carbon and energy source Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.84 0.0042
-
 reductase Pseudomonas putida
1.14.14.84 0.014
-
 purified enzyme, pH 7.4, temperature not specified in the publication Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.14.14.84 -196°C, LIN-redoxin reductase and cytochrome LINP-450 in solution stable over a long period after ultrafiltration or dialysis and repeated freeze/thaw-cycles Pseudomonas putida
1.14.14.84 0°C, LIN-redoxin loses its prosthetic group, 5 mM DTT retards apoprotein formation Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.84 (6E)-8-hydroxylinalool + [reduced NADPH-hemoprotein reductase] + O2
-
 Pseudomonas putida (6E)-8-oxolinalool + [oxidized NADPH-hemoprotein reductase] + H2O product analysis by mass spectrometry ?
1.14.14.84 (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [reduced NADPH-hemoprotein reductase] + O2 8-hydroxylinalool Pseudomonas putida 8-oxolinalool + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.84 (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [reduced NADPH-hemoprotein reductase] + O2 8-hydroxylinalool Pseudomonas putida PpG777 8-oxolinalool + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.84 3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Pseudomonas putida (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O 8-hydroxylinalool ?
1.14.14.84 3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2 linalool, initial reaction in linalool catabolism Pseudomonas putida (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.84 3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2
-
 Pseudomonas putida PpG777 (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O 8-hydroxylinalool ?
1.14.14.84 3,7-dimethylocta-1,6-dien-3-ol + [reduced NADPH-hemoprotein reductase] + O2 linalool, initial reaction in linalool catabolism Pseudomonas putida PpG777 (E)-3,7-dimethylocta-1,6-dien-3,8-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Pseudomonas putida (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
 ?
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 overall reaction Pseudomonas putida (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O product analysis by mass spectrometry ?
1.14.14.84 linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Pseudomonas putida PpG777 (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
 ?
1.14.14.84 linalool + NADH + O2
-
 Pseudomonas putida (6E)-8-hydroxylinalool + NAD+ + H2O
-
 r
1.14.14.84 linalool + [reduced NADPH-hemoprotein reductase] + O2
-
 Pseudomonas putida (6E)-8-hydroxylinalool + [oxidized NADPH-hemoprotein reductase] + H2O product analysis by mass spectrometry ?
1.14.14.84 additional information the enzyme does not perform 10-methyl hydroxylation of linalool Pseudomonas putida ?
-
 ?
1.14.14.84 additional information the enzyme does not perform 10-methyl hydroxylation of linalool Pseudomonas putida PpG777 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.84 monomer 1 * 44000, SDS-PAGE, 1 * 43700, about, sequence calculation Pseudomonas putida
1.14.14.84 More enzyme consists of LIN-reductase, MW 44000 Da, and Fe2S2-redoxin, MW 10700 Da, and cytochrome LIN-P450, MW 47000 Da, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.14.84 cytochrome P-450 linalool 8-hydroxylase
-
 Pseudomonas putida
1.14.14.84 cytochrome P-450-dependent linalool 8-hydroxylase
-
 Pseudomonas putida
1.14.14.84 LIN reductase
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.84 2
-
 NADH substrate-stimulated NADH oxidation using excess amounts of P-450 and FAD component of the enzyme Pseudomonas putida
1.14.14.84 32
-
 NADH substrate-stimulated NADH oxidation using excess amounts of iron-sulfur and FAD component of the enzyme Pseudomonas putida
1.14.14.84 43
-
 NADH substrate-stimulated NADH oxidation using excess amounts of iron sulfur and P-450 component of the enzyme Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.84 7.4
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.84 cytochrome P450
-
 Pseudomonas putida
1.14.14.84 heme
-
 Pseudomonas putida
1.14.14.84 redoxin an Fe2/S2/Cys4 protein Pseudomonas putida

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.14.84 Pseudomonas putida sequence calculation
-
 4.65

General Information

EC Number General Information Comment Organism
1.14.14.84 metabolism the enzyme catalyzes the first step in linalool catabolism Pseudomonas putida
1.14.14.84 additional information analysis of the heme-thiolate reaction center, molecular domains of substrate specificity, redox-pair interactions, and the regulation of the reaction cycle, overview Pseudomonas putida