Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vogel, H.J.; McLellan, W.L.
    N-Acetylglutamic gamma-semialdehyde dehydrogenase (Escherichia coli) (1970), Methods Enzymol., 17A, 255-260.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.2.1.38 gel filtration, enzyme unstable Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.38 5,5'-dithiobis(2-nitrobenzoate)
-
 Escherichia coli
1.2.1.38 Cu2+
-
 Escherichia coli
1.2.1.38 Fe2+ slight Escherichia coli
1.2.1.38 Mn2+ slight Escherichia coli
1.2.1.38 N-ethylmaleimide
-
 Escherichia coli
1.2.1.38 Ni2+ slight Escherichia coli
1.2.1.38 p-hydroxymercuribenzoate
-
 Escherichia coli
1.2.1.38 Zn2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.38 0.17
-
 NADP+
-
 Escherichia coli
1.2.1.38 0.6
-
 N-acetyl-L-glumate 5-semialdehyde
-
 Escherichia coli
1.2.1.38 3
-
 phosphate
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.38 N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate Escherichia coli enzyme of arginine biosynthesis N-acetyl-5-glutamyl phosphate + NADPH
-
 ?
1.2.1.38 N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate Escherichia coli enzyme of arginine biosynthesis N-acetyl-5-glutamyl phosphate + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.38 Escherichia coli
-
 W2D ATCC 25542
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.38
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.38 additional information
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.2.1.38 -15°C, several weeks, pH 7.0, 0.1 M phosphate, 1.0 mM 2-mercaptoethanol, 0.1 mM EDTA Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.38 N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate in the dehydrogenase reaction, arsenate can substitute for phosphate Escherichia coli N-acetyl-5-glutamyl phosphate + NADPH
-
 r
1.2.1.38 N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate enzyme of arginine biosynthesis Escherichia coli N-acetyl-5-glutamyl phosphate + NADPH
-
 ?
1.2.1.38 N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate enzyme of arginine biosynthesis Escherichia coli N-acetyl-5-glutamyl phosphate + NADPH
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.38 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.38 9.3
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.38 9 9.5 activity drops sharply below pH 9.0 and above 9.5 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.38 5.5 9.5 4°C, 5 h Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.38 NADP+
-
 Escherichia coli