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Literature summary extracted from

  • Hayzer, D.J.; Leisinger, T.
    Proline biosynthesis in Escherichia coli. Purification and characterisation of glutamate-semialdehyde dehydrogenase (1982), Eur. J. Biochem., 121, 561-565.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.41
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.41 Ba2+ 50% inhibition at 1 mM Escherichia coli
1.2.1.41 Co2+ 50% inhibition at 2.5 mM Escherichia coli
1.2.1.41 Cu2+ 75% inhibition at 0.1 mM Escherichia coli
1.2.1.41 iodoacetamide 40% inhibition at 1 mM Escherichia coli
1.2.1.41 iodoacetate 15% inhibition at 1 mM Escherichia coli
1.2.1.41 L-proline 25% inhibition at 50 mM Pseudomonas aeruginosa
1.2.1.41 Ni2+ 100% inhibition at 0.1 mM Escherichia coli
1.2.1.41 p-chloromercuribenzoate 100% inhibition at 1 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.41 47000
-
 SDS-PAGE, monomeric form Escherichia coli
1.2.1.41 189000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.41 Escherichia coli
-
-
-
1.2.1.41 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.41 homogeneity Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.41 13.84
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.2.1.41 -20°C, 6 months Escherichia coli
1.2.1.41 -70°C, 10% glycerol Escherichia coli
1.2.1.41 0-2°C, 2 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.41 L-glutamate 5-semialdehyde + phosphate + NADP+
-
 Escherichia coli L-5-glutamyl phosphate + NADPH
-
 ?
1.2.1.41 L-glutamate 5-semialdehyde + phosphate + NADP+
-
 Pseudomonas aeruginosa L-5-glutamyl phosphate + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.41 tetramer 4 * 47000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.41 50
-
 rapidly inactivated above, pH 7.0, 30 min, stable below Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.41 7
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.41 6 8.5 pH 6.0 and pH 8.5: about 20% of activity maximum Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.41 6 9 stable for at least 10 min Escherichia coli
1.2.1.41 7
-
 long periods Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.41 NADPH
-
 Escherichia coli
1.2.1.41 NADPH
-
 Pseudomonas aeruginosa