EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.169 | additional information | neither ATP nor phosphate stimulate the exchange | Moorella thermoacetica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.4 | 5,5'-dithiobis (2-nitrobenzoate) | - |
Moorella thermoacetica | |
1.2.7.4 | Mersalyl acid | - |
Moorella thermoacetica | |
1.2.7.4 | methyl iodide | - |
Moorella thermoacetica | |
1.2.7.4 | additional information | phenylglyoxal, methylglyoxal, butanedione, mersalyl acid, methyl iodide, 5,5'-dithiobis (2-nitrobenzoate) and sodium dithionite inhibits the exchange reaction between CO and acetyl-CoA | Moorella thermoacetica | |
2.3.1.169 | 5,5'dithiobis-(2-nitrobenzoic acid) | inhibits the acetyl-CoA/CO exchange reaction | Moorella thermoacetica | |
2.3.1.169 | Mersalyl acid | inhibits the acetyl-CoA/CO exchange reaction | Moorella thermoacetica | |
2.3.1.169 | methyl iodide | inhibits the acetyl-CoA/CO exchange reaction | Moorella thermoacetica | |
2.3.1.169 | additional information | no inhibition of the exchange reaction by methyl- and phenylglyoxal, and butanedione | Moorella thermoacetica | |
2.3.1.169 | Sodium dithionite | inhibits the acetyl-CoA/CO exchange reaction | Moorella thermoacetica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.4 | CO + H2O + electron acceptor | Moorella thermoacetica | key role of the enzyme in the synthesis of acetyl-CoA | ? | - |
? | |
1.2.7.4 | CO + H2O + oxidized ferredoxin | Moorella thermoacetica | acetate biosynthesis pathway, catalyzes an exchange reaction between CO and the carbonyl group of acetyl-CoA | CO2 + reduced ferredoxin | - |
? | |
2.3.1.169 | additional information | Moorella thermoacetica | the purified carbon monoxide dehydrogenase, EC 1.2.7.4, from Clostridium thermoaceticum is the only protein required to catalyze a reversible exchange reaction between carbon monoxide and the carbonyl group of acetyl-CoA. Carbon dioxide also exchanges with the C-1 of acetyl-coA, but at a much lower rate than does CO | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.4 | Moorella thermoacetica | - |
- |
- |
1.2.7.4 | Moorella thermoacetica | - |
formerly Clostridium thermoaceticum | - |
2.3.1.169 | Moorella thermoacetica | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.4 | - |
Moorella thermoacetica |
2.3.1.169 | native enzyme by anion exchange and hydrophobic interaction chromatography, followed by hydroxylapatite chromatography, gel filtration, and CoA affinity chromatography | Moorella thermoacetica |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.169 | additional information | - |
70 mol of CO exchanged per min/mol of enzyme | Moorella thermoacetica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.4 | CO + H2O + electron acceptor | key role of the enzyme in the synthesis of acetyl-CoA | Moorella thermoacetica | ? | - |
? | |
1.2.7.4 | CO + H2O + methyl viologen | catalyzes at a low rate an exchange between CO2 and the carbonyl group of acetyl-CoA | Moorella thermoacetica | CO2 + reduced methyl viologen | - |
? | |
1.2.7.4 | CO + H2O + methyl viologen | catalyzes an exchange reaction between CO and the carbonyl group of acetyl-CoA | Moorella thermoacetica | CO2 + reduced methyl viologen | - |
? | |
1.2.7.4 | CO + H2O + oxidized ferredoxin | - |
Moorella thermoacetica | CO2 + reduced ferredoxin | - |
? | |
1.2.7.4 | CO + H2O + oxidized ferredoxin | acetate biosynthesis pathway, catalyzes an exchange reaction between CO and the carbonyl group of acetyl-CoA | Moorella thermoacetica | CO2 + reduced ferredoxin | - |
? | |
2.3.1.169 | additional information | the purified carbon monoxide dehydrogenase, EC 1.2.7.4, from Clostridium thermoaceticum is the only protein required to catalyze a reversible exchange reaction between carbon monoxide and the carbonyl group of acetyl-CoA. Carbon dioxide also exchanges with the C-1 of acetyl-coA, but at a much lower rate than does CO | Moorella thermoacetica | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.169 | 50 | - |
- |
Moorella thermoacetica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.4 | additional information | - |
additional information | pH 5.3, 50°C, activity of CO oxidation to CO2, ferredoxin II as electron carrier, turnover number is approximately 780 mol/mol of CO dehydrogenase | Moorella thermoacetica | |
1.2.7.4 | 1.17 | - |
CO | turnover mumber for CO/CO2 exchange reaction | Moorella thermoacetica | |
1.2.7.4 | 13 | - |
CO | CO oxidation, 50°C, pH 5.3, electron acceptor ferredoxin II | Moorella thermoacetica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.7.4 | 5.3 | - |
- |
Moorella thermoacetica |
2.3.1.169 | 5.3 | - |
- |
Moorella thermoacetica |