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Literature summary extracted from
- Carbon monoxide dehydrogenase from Methanosarcina frisia Gö1 (1996), J. Biol. Chem., 271, 14256-14263.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.2.7.4 | - |
Methanosarcina mazei |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.7.4 | 0.12 | - |
methyl viologen | - |
Methanosarcina mazei |  |
| 1.2.7.4 | 0.9 | - |
CO | - |
Methanosarcina mazei | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.7.4 | Iron | contains 25 mol of Fe2+ and 20 mol of S2- per mol of tetramer, the enzyme contains 2 (4Fe-4S)+ clusters | Methanosarcina mazei | |
| 1.2.7.4 | Nickel | contains 1.3 mol of Ni2+ per mol of tetramer | Methanosarcina mazei | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.4 | 19000 | - |
2 * 19000 + 2 * 94000, alpha2beta2, SDS-PAGE | Methanosarcina mazei |
| 1.2.7.4 | 94000 | - |
2 * 19000 + 2 * 94000, alpha2beta2, SDS-PAGE | Methanosarcina mazei |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.7.4 | Methanosarcina mazei | - |
strain Gö1 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.7.4 | - |
Methanosarcina mazei |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.4 | 320 | - |
- |
Methanosarcina mazei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.7.4 | CO + H2O + methyl viologen | - |
Methanosarcina mazei | CO2 + reduced methyl viologen | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.2.7.4 | tetramer | 2 * 19000 + 2 * 94000, alpha2beta2, SDS-PAGE | Methanosarcina mazei |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.4 | 8 | 9 | - |
Methanosarcina mazei |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.7.4 | 6.5 | 9 | pH 6.5: about 25% of maximal activity, pH 8-9: maximal activity | Methanosarcina mazei |
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