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Literature summary extracted from

  • Dobbek, H.; Svetlitchnyi, V.; Gremer, L.; Huber, R.; Meyer, O.
    Crystal structure of a carbon monoxide dehydrogenase reveals a [Ni-4Fe-5S] cluster (2001), Science, 293, 1281-1285.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.7.4 carbon monoxide dehydrogenase II, hanging drop vapor diffusion, 20% 2-propanol, 20% polyethylene glycol 3000, 100 mM HEPES, pH 7.5 Carboxydothermus hydrogenoformans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.4 Nickel carbon monoxide dehydrogenase II, enzyme contains 5 metal clusters, a [Ni-4Fe-5S] cluster appears to be the active site of CO oxidation Carboxydothermus hydrogenoformans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.4 CO + H2O Carboxydothermus hydrogenoformans
-
 CO2 + H2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Carboxydothermus hydrogenoformans
-
 hydrogenogenic bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.4 carbon monoxide dehydrogenase II Carboxydothermus hydrogenoformans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.4 14000
-
 carbon monoxide dehydrogenase II Carboxydothermus hydrogenoformans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 CO + H2O
-
 Carboxydothermus hydrogenoformans CO2 + H2
-
 ?