Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fong, K.P.Y.; Tan, H.M.
    Characterization of a novel cis-benzene dihydrodiol dehydrogenase from Pseudomonas putida ML2 (1999), FEBS Lett., 451, 5-9.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.3.1.19 unstable Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.19 0.01
-
cis-1,2-dihydrobenzene-1,2-diol cosubstrate NAD+ Pseudomonas putida
1.3.1.19 0.22
-
NAD+
-
Pseudomonas putida
1.3.1.19 46
-
glycerol cosubstrate NAD+ Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.19 additional information no metal ions requirement Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.19 39000
-
4 * 39000, SDS-PAGE Pseudomonas putida
1.3.1.19 166000
-
PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas putida
-
catechol + NADH
-
?
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas putida ML2
-
catechol + NADH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.19 Pseudomonas putida
-
-
-
1.3.1.19 Pseudomonas putida ML2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.19 dye-affinity chromatography and NAD+ agarose chromatography Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.19 68
-
glycerol, NAD+ Pseudomonas putida
1.3.1.19 153
-
cis-1,2-dihydrobenzene-1,2-diol, NAD+ Pseudomonas putida
1.3.1.19 313
-
cis-1,2-cyclohexanediol, NAD+ Pseudomonas putida
1.3.1.19 513
-
1,2-propanediol, NAD+ Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.3.1.19 less stable at 4°C than at -20°C, loss of activity at 4°C gradually over a few days Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.19 1,2-propanediol + NAD+
-
Pseudomonas putida propen-1,2-diol + NADH
-
?
1.3.1.19 1,2-propanediol + NAD+
-
Pseudomonas putida ML2 propen-1,2-diol + NADH
-
?
1.3.1.19 cis-1,2-cyclohexanediol + NAD+
-
Pseudomonas putida cyclohex-1-ene-1,2-diol + NADH
-
?
1.3.1.19 cis-1,2-cyclohexanediol + NAD+
-
Pseudomonas putida ML2 cyclohex-1-ene-1,2-diol + NADH
-
?
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
Pseudomonas putida catechol + NADH
-
?
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
Pseudomonas putida ML2 catechol + NADH
-
?
1.3.1.19 glycerol + NAD+
-
Pseudomonas putida ?
-
?
1.3.1.19 glycerol + NAD+
-
Pseudomonas putida ML2 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.3.1.19 tetramer 4 * 39000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.3.1.19 cis-benzene dihydrodiol dehydrogenase bedD enzyme Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.19 30
-
enzyme assay Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.19 9
-
with NAD+ as cofactor, 100 mM potassium carbonate buffer Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.19 NAD+
-
Pseudomonas putida