Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Patel, T.R.; Gibson, D.T.
    Purification and properties of (plus)-cis-naphthalene dihydrodiol dehydrogenase of Pseudomonas putida (1974), J. Bacteriol., 119, 879-888.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.3.1.29 2-mercaptoethanol stabilizes Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.29 2,2'-dipyridyl 16.7 mM, 55% inhibition Pseudomonas putida
1.3.1.29 8-hydroxyquinoline 16.7 mM, 25% inhibition Pseudomonas putida
1.3.1.29 EDTA 16.7 mM, 17% inhibition Pseudomonas putida
1.3.1.29 p-chloromercuribenzoate 16.7 mM, 87% inhibition Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.29 0.029
-
 NAD+
-
 Pseudomonas putida
1.3.1.29 0.8
-
 cis-1,2-dihydronaphthalene-1,2-diol
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.29 25500
-
 4 * 25500, SDS-PAGE Pseudomonas putida
1.3.1.29 102000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.29 Pseudomonas putida
-
 strains NP, PL6 and C84-15
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.29
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.29 additional information
-
-
 Pseudomonas putida
1.3.1.29 8.43
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.3.1.29 4°C, 11 days, little loss of activity Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.29 cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ i.e. cis-naphthalene dihydrodiol, specific for (+)-isomers Pseudomonas putida 1,2-dihydroxynaphthalene + NADH
-
 ?
1.3.1.29 cis-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 Pseudomonas putida naphthalene-1,2-diol + NADH + H+
-
 r
1.3.1.29 cis-anthracene dihydrodiol + NAD+ 56% of activity with cis-naphthalene dihydrodiol Pseudomonas putida 1,2-dihydroxyanthracene + NADH
-
 ?
1.3.1.29 cis-benzene dihydrodiol + NAD+ 4% of activity with cis-naphthalene dihydrodiol Pseudomonas putida 1,2-dihydroxybenzene + NADH
-
 ?
1.3.1.29 cis-biphenyl dihydrodiol + NAD+ 43% of activity with cis-naphthalene dihydrodiol Pseudomonas putida 3-phenyl-1,2-dihydroxybenzene + NADH
-
 ?
1.3.1.29 cis-ethylbenzene dihydrodiol + NAD+ 48% of activity with cis-naphthalene dihydrodiol Pseudomonas putida 3-ethyl-1,2-dihydroxybenzene + NADH
-
 ?
1.3.1.29 cis-phenanthrene dihydrodiol + NAD+ 53% of activity with cis-naphtalene dihydrodiol Pseudomonas putida 3,4-dihydroxyphenanthrene + NADH
-
 ?
1.3.1.29 cis-toluene dihydrodiol + NAD+ 26% of activity with cis-naphthalene dihydrodiol Pseudomonas putida 3-methyl-1,2-dihydroxybenzene + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.29 tetramer 4 * 25500, SDS-PAGE Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.29 8.8 9
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.29 NAD+ absolutely required Pseudomonas putida