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Literature summary extracted from

  • Kuno, S.; Bacher, A.; Simon, H.
    Structure of enoate reductase from a Clostridium tyrobutyricum (C. spec. La1) (1985), Biol. Chem. Hoppe-Seyler, 366, 463-472.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.31 Fe iron-sulfur protein, contains 4.7 mol Fe per mol of subunit Clostridium tyrobutyricum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.31 70000
-
 12 * 70000, SDS-PAGE Clostridium tyrobutyricum
1.3.1.31 73000
-
 12 * 73000, equilibrium sedimentation in guanidine hydrochloride Clostridium tyrobutyricum
1.3.1.31 800000
-
 gel filtration Clostridium tyrobutyricum
1.3.1.31 940000
-
 equilibrium ultracentrifugation Clostridium tyrobutyricum

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.31 Clostridium tyrobutyricum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.31
-
 Clostridium tyrobutyricum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.31 10.6
-
-
 Clostridium tyrobutyricum

Storage Stability

EC Number Storage Stability Organism
1.3.1.31 -18°C, pH 7, buffer containing 10 mM phosphate and 0.1 M KCl, formation of a dissociation product sedimenting at about 10 S Clostridium tyrobutyricum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.31 butenoate + NADH
-
 Clostridium tyrobutyricum butanoate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.31 dodecamer 12 * 70000, SDS-PAGE Clostridium tyrobutyricum
1.3.1.31 dodecamer 12 * 73000, equilibrium sedimentation in guanidine hydrochloride Clostridium tyrobutyricum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.31 NADH
-
 Clostridium tyrobutyricum