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Literature summary extracted from

  • Kaschabek, S.R.; Reineke, W.
    Degradation of chloroaromatics: purification and characterization of maleylacetate reductase from Pseudomonas sp. strain B13 (1993), J. Bacteriol., 175, 6075-6081.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.32
-
 Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.32 Ag+
-
 Pseudomonas sp.
1.3.1.32 AgNO3
-
 Pseudomonas sp.
1.3.1.32 Cu2+
-
 Pseudomonas sp.
1.3.1.32 CuSO4
-
 Cutaneotrichosporon cutaneum
1.3.1.32 CuSO4
-
 Pseudomonas sp.
1.3.1.32 Hg2+
-
 Pseudomonas sp.
1.3.1.32 HgCl2
-
 Pseudomonas sp.
1.3.1.32 p-chloromercuribenzoate
-
 Cupriavidus necator
1.3.1.32 p-chloromercuribenzoate
-
 Cutaneotrichosporon cutaneum
1.3.1.32 p-chloromercuribenzoate
-
 Pseudomonas sp.
1.3.1.32 Zn2+
-
 Pseudomonas sp.
1.3.1.32 ZnCl2
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.32 0.03
-
 NADH
-
 Pseudomonas sp.
1.3.1.32 0.058
-
 maleylacetate
-
 Pseudomonas sp.
1.3.1.32 0.076
-
 2-chloromaleylacetate
-
 Pseudomonas sp.
1.3.1.32 0.077
-
 NADPH
-
 Pseudomonas sp.
1.3.1.32 0.308
-
 2-methylmaleylacetate
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.32 37000
-
 2 * 37000, SDS-PAGE Pseudomonas sp.
1.3.1.32 74000
-
-
 Pseudomonas aeruginosa
1.3.1.32 74000
-
-
 Cutaneotrichosporon cutaneum
1.3.1.32 74000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.32 2-maleylacetate + NADPH Pseudomonas aeruginosa
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Pseudomonas sp.
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Cupriavidus necator
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Delftia acidovorans
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Cutaneotrichosporon cutaneum
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Delftia acidovorans CA28
-
 3-oxoadipate + NADP+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.32 Cupriavidus necator
-
 JMP 134
-
1.3.1.32 Cupriavidus necator JMP 134-1
-
 JMP 134
-
1.3.1.32 Cutaneotrichosporon cutaneum
-
-
-
1.3.1.32 Delftia acidovorans
-
 CA28
-
1.3.1.32 Delftia acidovorans CA28
-
 CA28
-
1.3.1.32 Pseudomonas aeruginosa
-
-
-
1.3.1.32 Pseudomonas aeruginosa RHO1
-
-
-
1.3.1.32 Pseudomonas sp.
-
-
-
1.3.1.32 Pseudomonas sp. B13
-
-
-
1.3.1.32 Pseudomonas sp. JS100
-
-
-
1.3.1.32 Pseudomonas sp. JS6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.32
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.32 582.9
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.3.1.32 -20°C, 100 mM Tris/HCl buffer, pH 7.0, containing 50% (vol/vol) glycerol, 1 mM dithioerythritol and 0.5 mM EDTA, purified enzyme retains 80% of its initial activity after storage for 2 months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.32 2-chloromaleylacetate + NADH
-
 Pseudomonas aeruginosa 2-maleylacetate + Cl- + NAD+
-
 ?
1.3.1.32 2-chloromaleylacetate + NADH
-
 Pseudomonas sp. 2-maleylacetate + Cl- + NAD+
-
 ?
1.3.1.32 2-chloromaleylacetate + NADH
-
 Cupriavidus necator 2-maleylacetate + Cl- + NAD+
-
 ?
1.3.1.32 2-chloromaleylacetate + NADH
-
 Pseudomonas sp. B13 2-maleylacetate + Cl- + NAD+
-
 ?
1.3.1.32 2-chloromaleylacetate + NADH
-
 Pseudomonas sp. JS100 2-maleylacetate + Cl- + NAD+
-
 ?
1.3.1.32 2-chloromaleylacetate + NADH
-
 Pseudomonas aeruginosa RHO1 2-maleylacetate + Cl- + NAD+
-
 ?
1.3.1.32 2-chloromaleylacetate + NADH
-
 Pseudomonas sp. JS6 2-maleylacetate + Cl- + NAD+
-
 ?
1.3.1.32 2-chloromaleylacetate + NADH
-
 Cupriavidus necator JMP 134-1 2-maleylacetate + Cl- + NAD+
-
 ?
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas sp. 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Cupriavidus necator 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Delftia acidovorans 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Cutaneotrichosporon cutaneum 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas sp. B13 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Delftia acidovorans CA28 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas sp. JS100 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa RHO1 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas sp. JS6 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Cupriavidus necator JMP 134-1 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Pseudomonas aeruginosa 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Pseudomonas sp. 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Cupriavidus necator 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Delftia acidovorans 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Cutaneotrichosporon cutaneum 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Delftia acidovorans CA28 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-methylmaleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa 5-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 2-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. 5-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 2-methylmaleylacetate + NAD(P)H
-
 Cupriavidus necator 5-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 2-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. B13 5-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 2-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. JS100 5-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 2-methylmaleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa RHO1 5-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 2-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. JS6 5-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 2-methylmaleylacetate + NAD(P)H
-
 Cupriavidus necator JMP 134-1 5-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 3-methylmaleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa 4-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 3-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. 4-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 3-methylmaleylacetate + NAD(P)H
-
 Cupriavidus necator 4-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 3-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. B13 4-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 3-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. JS100 4-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 3-methylmaleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa RHO1 4-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 3-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. JS6 4-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 3-methylmaleylacetate + NAD(P)H
-
 Cupriavidus necator JMP 134-1 4-methyl-3-oxoadipate + NAD(P)+
-
 ?
1.3.1.32 5-methylmaleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa 2-methyl-3-oxoadipate + NADP+
-
 ?
1.3.1.32 5-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. 2-methyl-3-oxoadipate + NADP+
-
 ?
1.3.1.32 5-methylmaleylacetate + NAD(P)H
-
 Cupriavidus necator 2-methyl-3-oxoadipate + NADP+
-
 ?
1.3.1.32 5-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. B13 2-methyl-3-oxoadipate + NADP+
-
 ?
1.3.1.32 5-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. JS100 2-methyl-3-oxoadipate + NADP+
-
 ?
1.3.1.32 5-methylmaleylacetate + NAD(P)H
-
 Pseudomonas aeruginosa RHO1 2-methyl-3-oxoadipate + NADP+
-
 ?
1.3.1.32 5-methylmaleylacetate + NAD(P)H
-
 Pseudomonas sp. JS6 2-methyl-3-oxoadipate + NADP+
-
 ?
1.3.1.32 5-methylmaleylacetate + NAD(P)H
-
 Cupriavidus necator JMP 134-1 2-methyl-3-oxoadipate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.32 dimer
-
 Pseudomonas aeruginosa
1.3.1.32 dimer
-
 Cupriavidus necator
1.3.1.32 dimer
-
 Cutaneotrichosporon cutaneum
1.3.1.32 dimer 2 * 37000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.32 50
-
-
 Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.1.32 5 60
-
 Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.32 13.9
-
 maleylacetate
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.32 5.4
-
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.32 NADH
-
 Pseudomonas sp.
1.3.1.32 NADH
-
 Cupriavidus necator
1.3.1.32 NADH
-
 Cutaneotrichosporon cutaneum
1.3.1.32 NADPH
-
 Pseudomonas sp.
1.3.1.32 NADPH
-
 Cupriavidus necator
1.3.1.32 NADPH
-
 Cutaneotrichosporon cutaneum