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Literature summary extracted from

  • Maitra, S.K.; Kumar, S.
    Crotonyl coenzyme A reductase activity of bovine mammary fatty acid synthetase (1974), J. Biol. Chem., 249, 111-117.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.8 dithiothreitol enhancement in the range of 5-10 mM Bos taurus

General Stability

EC Number General Stability Organism
1.3.1.86 the crotonyl-CoA reductase activity is influenced by preincubation. 0.25 M Tris-HCl buffer diminishes the crotonyl-CoA reductase activity by approximately 30% Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.8 dithiothreitol above 10 mM Bos taurus
1.3.1.86 2-mercaptoethanol at high concentrations beta-mercaptoethanol is inhibitory Bos taurus
1.3.1.86 dithiothreitol at high concentrations dithiothreitol is inhibitory Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.8 0.0033
-
 trans-crotonyl-CoA
-
 Bos taurus
1.3.1.8 0.0067
-
 butyryl-CoA
-
 Bos taurus
1.3.1.8 0.13
-
 acetyl-CoA
-
 Bos taurus
1.3.1.86 0.0033
-
 trans-crotonyl-CoA in 200 mM potassium phosphate buffer, pH 6.8, at 26°C Bos taurus
1.3.1.86 0.00364
-
 NADPH in 200 mM potassium phosphate buffer, pH 6.8, at 26°C Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.8 Bos taurus
-
-
-
1.3.1.86 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.8 fatty acid synthetase, also exhibiting crotonyl-CoA reductase activity Bos taurus
1.3.1.86 ammonium sulfate precipitation, calcium phosphate gel treatment, alumina gel Cgamma extract filtration, Sephadex G-200 gel filtration Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.8 mammary gland
-
 Bos taurus
-
1.3.1.86 mammary gland
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.86 0.0061
-
 crude extract, pH 6.8, at 26°C Bos taurus
1.3.1.86 0.0501
-
 after 11.2fold purification, pH 6.8, at 26°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.8 acetyl-CoA + NADPH + H+
-
 Bos taurus ?
-
 r
1.3.1.8 crotonyl-CoA + NAD(P)H
-
 Bos taurus butanoyl-CoA + NADP+
-
 ?
1.3.1.86 additional information the crotonyl-CoA reductase reaction requires NADPH as electron donor, but at a 20fold higher concentration NADH will substitute for NADPH with 50% Vmax Bos taurus ?
-
 ?
1.3.1.86 trans-crotonyl-CoA + NADPH + H+ the crotonyl-CoA reductase reaction requires NADPH as electron donor Bos taurus butyryl-CoA + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.86 crotonyl coenzyme A reductase
-
 Bos taurus
1.3.1.86 trans-crotonyl CoA reductase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.8 37
-
-
 Bos taurus
1.3.1.86 37
-
-
 Bos taurus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.1.8 30 42
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.8 7.2 8.5
-
 Bos taurus
1.3.1.86 7 8.5
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.8 NADH 20fold higher concentration of NADH compared to NADPH results in 50% of NADPH activity Bos taurus
1.3.1.8 NADPH
-
 Bos taurus
1.3.1.86 NADPH the crotonyl-CoA reductase reaction requires NADPH as electron donor, but at a 20fold higher concentration NADH will substitute for NADPH with 50% Vmax Bos taurus