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Literature summary extracted from

  • Yabuta, Y.; Yoshimura, K.; Takeda, T.; Shigeoka, S.
    Molecular characterization of tobacco mitochondrial L-galactono-gamma-lactone dehydrogenase and its expression in Escherichia coli (2000), Plant Cell Physiol., 41, 666-675.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.2.3 expression in Escherichia coli Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.2.3 Acriflavine diminished by addition of FAD or FMN Nicotiana tabacum
1.3.2.3 Cu2+ strong inhibition Nicotiana tabacum
1.3.2.3 N-ethylmaleimide complete inhibition Nicotiana tabacum
1.3.2.3 p-chloromercuribenzoate
-
 Nicotiana tabacum
1.3.2.3 Zn2+ strong inhibition Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.2.3 mitochondrion soluble Nicotiana tabacum 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.2.3 56000
-
 recombinant enzyme, sequence determination Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.2.3 L-galactono-1,4-lactone + 2 ferricytochrome c Nicotiana tabacum
-
 L-ascorbate + 2 ferrocytochrome c + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.2.3 Nicotiana tabacum
-
 tobacco
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.2.3 partial, wild-type and recombinant from Escherichia coli Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.2.3 leaf
-
 Nicotiana tabacum
-
1.3.2.3 root
-
 Nicotiana tabacum
-
1.3.2.3 stem
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.2.3 L-galactono-1,4-lactone + 2 ferricytochrome c
-
 Nicotiana tabacum L-ascorbate + 2 ferrocytochrome c + 2 H+
-
 ?
1.3.2.3 L-galactono-1,4-lactone + ferricytochrome c native and recombinant enzyme Nicotiana tabacum L-ascorbate + ferrocytochrome c
-
 ?
1.3.2.3 L-galactono-1,4-lactone + ferricytochrome c specific for L-galactono-gamma-lactone Nicotiana tabacum L-ascorbate + ferrocytochrome c
-
 ?
1.3.2.3 L-gulono-gamma-lactone + ferricytochrome recombinant enzyme: 7% activity of the activity with L-galactono-gamma-lactone Nicotiana tabacum D-ascorbate + ferricytochrome c
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.2.3 monomer 1 * 56000, SDS-PAGE Nicotiana tabacum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.2.3 7 9
-
 Nicotiana tabacum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.2.3 flavin
-
 Nicotiana tabacum