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Literature summary extracted from
- Catalytic properties of dihydroorotate dehydrogenase from Saccharomyces cerevisiae: studies on pH, alternate substrates, and inhibitors (2000), Arch. Biochem. Biophys., 378, 84-92.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.98.1 | 2-thioorotate | orotate analogue, 50% inhibition at 0.018 mM | Saccharomyces cerevisiae |  |
| 1.3.98.1 | 5-aminoorotate | orotate analogue, 50% inhibition at 1.6 mM | Saccharomyces cerevisiae | |
| 1.3.98.1 | 5-bromoorotate | orotate analogue, 50% inhibition at 0.016 mM | Saccharomyces cerevisiae | |
| 1.3.98.1 | 5-Fluoroorotate | orotate analogue, 50% inhibition at 0.055 mM | Saccharomyces cerevisiae | |
| 1.3.98.1 | 5-nitroorotate | orotate analogue, 50% inhibition at 0.076 mM | Saccharomyces cerevisiae | |
| 1.3.98.1 | Orotate | competitive, with ferricyanide as electron acceptor | Saccharomyces cerevisiae | |
| 1.3.98.1 | orotate methyl ester | orotate analogue, 50% inhibition at 0.71 mM | Saccharomyces cerevisiae | |
| 1.3.98.1 | oxonic acid | orotate analogue, 50% inhibition at 0.0099 mM | Saccharomyces cerevisiae | |
| 1.3.98.1 | Uracil | orotate analogue, 50% inhibition at 2.5 mM | Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.98.1 | 0.003 | - |
S-dihydroorotate | with O2 | Saccharomyces cerevisiae | |
| 1.3.98.1 | 0.0084 | 0.045 | S-dihydroorotate | with fumarate | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.98.1 | 35000 | - |
2 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
| 1.3.98.1 | 72000 | - |
sucrose-density-gradient centrifugation | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.98.1 | (S)-dihydroorotate + acceptor | Saccharomyces cerevisiae | fourth step in synthesis of pyrimidine nucleotides | orotate + reduced acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.98.1 | Saccharomyces cerevisiae | - |
- |
- |
| 1.3.98.1 | Saccharomyces cerevisiae | - |
family 1 enzyme A | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.98.1 | - |
Saccharomyces cerevisiae |
| 1.3.98.1 | Orange-A column, reverse-affinity step, Mono Q column | Saccharomyces cerevisiae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.3.98.1 | -20°C, 50% glycerol | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.98.1 | (S)-dihydroorotate + acceptor | preferred electron acceptors in the decreasing order: ferricyanide, 2,6-dichlorophenolindophenol, ubiquinone-0, fumarate and O2 | Saccharomyces cerevisiae | orotate + reduced acceptor | - |
? | |
| 1.3.98.1 | (S)-dihydroorotate + acceptor | fourth step in synthesis of pyrimidine nucleotides | Saccharomyces cerevisiae | orotate + reduced acceptor | - |
? | |
| 1.3.98.1 | dihydroorotate + acceptor | acceptor: 2,6-dichlorophenolindophenol | Saccharomyces cerevisiae | orotate + reduced acceptor | - |
? | |
| 1.3.98.1 | dihydroorotate + acceptor | kinetic isotope effects | Saccharomyces cerevisiae | orotate + reduced acceptor | - |
? | |
| 1.3.98.1 | dihydroorotate + acceptor | acceptor: ferricyanide, coenzyme Q0, fumarate, molecular oxygen | Saccharomyces cerevisiae | orotate + reduced acceptor | - |
? | |
| 1.3.98.1 | dihydrooxonate + acceptor | - |
Saccharomyces cerevisiae | oxonate + reduced acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.3.98.1 | dimer | 2 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.3.98.1 | DHOD | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.98.1 | 6.8 | - |
S-dihydroorotate | with O2 | Saccharomyces cerevisiae | |
| 1.3.98.1 | 23 | 25 | S-dihydroorotate | with fumarate, pH 7.5 | Saccharomyces cerevisiae | |
| 1.3.98.1 | 39 | - |
dihydrooxonate | with ferricyanide | Saccharomyces cerevisiae | |
| 1.3.98.1 | 190 | - |
S-dihydroorotate | with ferricyanide | Saccharomyces cerevisiae | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.98.1 | FMN | flavoprotein, 1 FMN per subunit | Saccharomyces cerevisiae | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.98.1 | 0.0102 | - |
Orotate | pH 8.0 | Saccharomyces cerevisiae | |
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