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Literature summary extracted from

  • Krungkrai, J.
    Purification, characterization and localization of mitochondrial dihydroorotate dehydrogenase in Plasmodium falciparum, human malaria parasite (1995), Biochim. Biophys. Acta, 1243, 351-360.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.2 5-aminoorotate
-
 Plasmodium falciparum
1.3.5.2 5-bromoorotate
-
 Plasmodium falciparum
1.3.5.2 5-Fluoroorotate
-
 Plasmodium falciparum
1.3.5.2 5-iodoorotate
-
 Plasmodium falciparum
1.3.5.2 5-Methylorotate
-
 Plasmodium falciparum
1.3.5.2 atovaquone
-
 Plasmodium falciparum
1.3.5.2 benzoquinones ubiquinone-0 and decylubiquinone Plasmodium falciparum
1.3.5.2 chloronaphthoquinone
-
 Plasmodium falciparum
1.3.5.2 Lapachol
-
 Plasmodium falciparum
1.3.5.2 Lawsone
-
 Plasmodium falciparum
1.3.5.2 menadione
-
 Plasmodium falciparum
1.3.5.2 Orotate also inhibitors: 5-substituted orotates; competitive to dihydroorotate; strong competitive inhibitor Plasmodium falciparum
1.3.5.2 rhodamine 123 mitochondrial inhibitor Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.2 0.014
-
 dihydroorotate
-
 Plasmodium falciparum
1.3.5.2 0.0144
-
 S-dihydroorotate
-
 Plasmodium falciparum
1.3.5.2 0.022
-
 coenzyme Q6
-
 Plasmodium falciparum
1.3.5.2 0.0225
-
 ubiquinone-6
-
 Plasmodium falciparum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.2 mitochondrion
-
 Plasmodium falciparum 5739
-
1.3.5.2 mitochondrion inner membrane Plasmodium falciparum 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.2 56000
-
 gel filtration Plasmodium falciparum
1.3.5.2 56000
-
 gel filtration, SDS-PAGE Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.2 (S)-dihydroorotate + ubiquinone Plasmodium falciparum fourth step in synthesis of uridine 5'-monophosphate orotate + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.2 Plasmodium falciparum
-
-
-
1.3.5.2 Plasmodium falciparum
-
 mature trophozytes and schizonts
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.2
-
 Plasmodium falciparum
1.3.5.2 cation-, anion-exchange, gel filtration Plasmodium falciparum

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.5.2 0.0002672
-
 37°C Plasmodium falciparum

Storage Stability

EC Number Storage Stability Organism
1.3.5.2 -80°C, addition of S-dihydroorotate to stabilize the enzyme activity Plasmodium falciparum
1.3.5.2 4°C, extremely labile, 90% decrease of activity over night Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.2 (S)-dihydroorotate + ubiquinone ubiquinone-6, ubiquinone-7, ubiquinone-9 and ubiquinone-10 maximize enzyme activity Plasmodium falciparum orotate + ubiquinol
-
 ?
1.3.5.2 (S)-dihydroorotate + ubiquinone fourth step in synthesis of uridine 5'-monophosphate Plasmodium falciparum orotate + ubiquinol
-
 ?
1.3.5.2 dihydroorotate + acceptor acceptor: quinones Plasmodium falciparum orotate + reduced acceptor
-
 ?
1.3.5.2 L-dihydroorotate + ubiquinone-6
-
 Plasmodium falciparum orotate + reduced ubiquinone-6
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.5.2 monomer 1 * 56000, SDS-PAGE Plasmodium falciparum
1.3.5.2 monomer SDS-PAGE Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.5.2 0.25
-
 S-dihydroorotate
-
 Plasmodium falciparum
1.3.5.2 0.36
-
 ubiquinone6
-
 Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.2 8
-
-
 Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.5.2 0.0151
-
 5-Fluoroorotate
-
 Plasmodium falciparum
1.3.5.2 0.018
-
 Orotate
-
 Plasmodium falciparum
1.3.5.2 0.0182
-
 Orotate
-
 Plasmodium falciparum
1.3.5.2 0.0244
-
 5-aminoorotate
-
 Plasmodium falciparum
1.3.5.2 0.1185
-
 5-Methylorotate
-
 Plasmodium falciparum
1.3.5.2 0.1408
-
 5-bromoorotate
-
 Plasmodium falciparum
1.3.5.2 0.2246
-
 5-iodoorotate
-
 Plasmodium falciparum