EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.20 | squalene and sterol carrier protein | 2fold stimulation of enzyme activity in microsomes, stimulation is lower if microsomes are pretreated with phospholipase A2, acetone powder, 0.05% sodium deoxycholate or 0.2% Triton X-100 | Rattus norvegicus |
EC Number | General Stability | Organism |
---|---|---|
1.14.19.20 | extremely labile | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.20 | 1,10-phenanthroline | 0.5 mM, 74% inhibition | Rattus norvegicus | |
1.14.19.20 | 4,5-dihydroxy-1,3-benzene-disulfonic acid | trivial name tiron, 0.5 mM, 80% inhibition | Rattus norvegicus | |
1.14.19.20 | bathophenanthroline | 0.5 mM, 71% inhibition | Rattus norvegicus | |
1.14.19.20 | CN- | 0.5 mM, 95% inhibition | Rattus norvegicus | |
1.14.19.20 | dithiothreitol | 1.0 mM, 55% inhibition | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.19.20 | 0.0357 | - |
5alpha-Cholest-7-en-3beta-ol | reconstituted enzyme system consisting of: cytochrome b5, cytochrome b5 reductase and egg lecithin liposomes | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.19.20 | microsome | - |
Rattus norvegicus | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.20 | 5alpha-cholest-7-en-3beta-ol + 2 ferrocytochrome b5 + O2 + 2 H+ | Rattus norvegicus | cholesterol biosynthesis | cholesta-5,7-dien-3beta-ol + 2 ferricytochrome b5 + 2 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.20 | Rattus norvegicus | - |
multienzyme system | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.19.20 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.19.20 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.19.20 | 0.0079 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.19.20 | 0°C, 24 h, 30% loss of activity | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.20 | 5alpha-cholest-7-en-3beta-ol + 2 ferrocytochrome b5 + O2 + 2 H+ | i.e. lathosterol | Rattus norvegicus | cholesta-5,7-dien-3beta-ol + 2 ferricytochrome b5 + 2 H2O | - |
? | |
1.14.19.20 | 5alpha-cholest-7-en-3beta-ol + 2 ferrocytochrome b5 + O2 + 2 H+ | enzyme is rather a mixed function oxidase which requires O2 and NAD(P)H for activity, than a dehydrogenase | Rattus norvegicus | cholesta-5,7-dien-3beta-ol + 2 ferricytochrome b5 + 2 H2O | - |
? | |
1.14.19.20 | 5alpha-cholest-7-en-3beta-ol + 2 ferrocytochrome b5 + O2 + 2 H+ | NADPH, 40% of activity with NADH | Rattus norvegicus | cholesta-5,7-dien-3beta-ol + 2 ferricytochrome b5 + 2 H2O | - |
? | |
1.14.19.20 | 5alpha-cholest-7-en-3beta-ol + 2 ferrocytochrome b5 + O2 + 2 H+ | cholesterol biosynthesis | Rattus norvegicus | cholesta-5,7-dien-3beta-ol + 2 ferricytochrome b5 + 2 H2O | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.20 | cytochrome b5 | required as electron carrier | Rattus norvegicus | |
1.14.19.20 | NAD(P)H | - |
Rattus norvegicus |