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Literature summary extracted from

  • Tushurashvili, P.R.; Gavrikova, E.V.; Ledenev, A.N.; Vinogradov, A.D.
    Studies on the succinate dehydrogenating system. Isolation and properties of the mitochondrial succinate-ubiquinone reductase (1985), Biochim. Biophys. Acta, 809, 145-159.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.1 2-Thenoyltrifluoroacetone
-
 Bos taurus
1.3.5.1 carboxin Q2-mediated Wurster's blue reduction: 90% inhibition at 0.02 mM, Q0-mediated Wurster's blue reduction: 75% inhibition at 0.02 mM, Q1-mediated Wurster's blue reduction: 88% inhibition at 0.02 mM, Q6-mediated Wurster's blue reduction: 50% inhibition at 0.02 mM Bos taurus
1.3.5.1 fumarate
-
 Bos taurus
1.3.5.1 malonate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.1 0.0005
-
 ubiquinone-2
-
 Bos taurus
1.3.5.1 0.02
-
 succinate
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.5.1 Fe
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Bos taurus
-
 succinate dehydrogenase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.1 using urea washing, Triton X-100 extraction, cholate-ammonium sulfate extraction, calcium phosphate gel and ammonium sulfate precipitation Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.5.1 heart
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.5.1 28 54 depending on assay temperature Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.3.5.1 liquid N2 or -20°C, pH 6.0, presence of 0.1% Triton X-100 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 succinate + 2,3-dimethoxy-5-methyl-6-geranyl-1,4-benzoquinone
-
 Bos taurus fumarate + 2,3-dimethoxy-5-methyl-6-geranyl-1,4-benzoquinol
-
 ?
1.3.5.1 succinate + ubiquinone
-
 Bos taurus fumarate + ubiquinol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.5.1 tetramer heart, succinate dehydrogenase, SDS-PAGE Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.5.1 83.3 150 succinate based on FAD content, dependency on assay temperature Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.1 6
-
-
 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.5.1 5 8 inactivation below pH 5, stable for about 20 h without loss of activity at pH 6.0 in the presence of 0.1% Triton X-100 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.1 cytochrome b
-
 Bos taurus
1.3.5.1 cytochrome b-560
-
 Bos taurus
1.3.5.1 FAD
-
 Bos taurus
1.3.5.1 additional information the enzyme contains acid-labile sulfides Bos taurus
1.3.5.1 ubiquinone
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.5.1 0.00025
-
 malonate
-
 Bos taurus
1.3.5.1 0.001
-
 carboxin
-
 Bos taurus
1.3.5.1 0.008
-
 2-Thenoyltrifluoroacetone
-
 Bos taurus
1.3.5.1 0.04
-
 fumarate
-
 Bos taurus