EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.1 | 0.27 | - |
phenazine methosulfate | - |
Rattus norvegicus | |
1.3.8.4 | 0.00043 | - |
FAD | - |
Rattus norvegicus | |
1.3.8.4 | 0.51 | - |
phenazine methosulfate | - |
Rattus norvegicus | |
1.3.8.5 | 0.00051 | - |
FAD | - |
Rattus norvegicus | |
1.3.8.5 | 0.48 | - |
phenazine methosulfate | - |
Rattus norvegicus | |
1.3.8.7 | 0.0024 | - |
FAD | - |
Rattus norvegicus | |
1.3.8.7 | 1.47 | - |
octanoyl-CoA | with phenazine methosulfate | Rattus norvegicus | |
1.3.8.8 | 0.0014 | - |
FAD | - |
Rattus norvegicus | |
1.3.8.8 | 1.1 | - |
phenazine methosulfate | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.8.1 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
1.3.8.4 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
1.3.8.5 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
1.3.8.8 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.4 | isovaleryl-CoA + acceptor | Rattus norvegicus | - |
3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
1.3.8.5 | 2-methylbutanoyl-CoA + electron transfer flavoprotein | Rattus norvegicus | - |
2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.8.1 | Rattus norvegicus | - |
- |
- |
1.3.8.4 | Rattus norvegicus | - |
- |
- |
1.3.8.5 | Rattus norvegicus | - |
- |
- |
1.3.8.7 | Rattus norvegicus | - |
- |
- |
1.3.8.8 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.8.4 | using sonication, ammonium sulfate treatment, column chromatography on DEAE-Sephadex A-50, hydroxylapatite and isoelectrofocusing | Rattus norvegicus |
1.3.8.5 | - |
Rattus norvegicus |
1.3.8.7 | - |
Rattus norvegicus |
1.3.8.8 | 35-80% ammonium sulfate precipitation, DEAE-Sephadex A-50, hydroxyapatite | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.8.1 | liver | - |
Rattus norvegicus | - |
1.3.8.4 | liver | - |
Rattus norvegicus | - |
1.3.8.5 | liver | - |
Rattus norvegicus | - |
1.3.8.7 | liver | - |
Rattus norvegicus | - |
1.3.8.8 | liver | - |
Rattus norvegicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.1 | butyryl-CoA + electron acceptor | terminal electron acceptor 2,6-dichlorophenol-indophenol | Rattus norvegicus | 2-butenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | butyryl-CoA + electron acceptor | intermediate electron acceptor phenazine methosulfate | Rattus norvegicus | 2-butenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | hexanoyl-CoA + phenazine methosulfate + dichloroindophenol | 13% of butyryl-CoA activity | Rattus norvegicus | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | pentanoyl-CoA + phenazine methosulfate | pentanoyl-CoA i.e. valeryl-CoA | Rattus norvegicus | 2-pentenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.4 | isovaleryl-CoA + acceptor | - |
Rattus norvegicus | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
1.3.8.4 | isovaleryl-CoA + acceptor | isovaleryl-CoA is the preferred substrate | Rattus norvegicus | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
1.3.8.4 | isovaleryl-CoA + acceptor | acceptors: electron-transfer flavoprotein, phenazine methosulfate | Rattus norvegicus | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
1.3.8.4 | isovaleryl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 3-methylcrotonyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.4 | n-valeryl-CoA + acceptor | - |
Rattus norvegicus | pent-2-enoyl-CoA + reduced acceptor | - |
? | |
1.3.8.5 | (S)-2-methylbutanoyl-CoA + acceptor | acceptors: electron transfer flavoprotein, phenazine methosulfate, meldola's blue | Rattus norvegicus | 2-methylbut-2-enoyl-CoA + reduced acceptor | - |
r | |
1.3.8.5 | 2-methylbutanoyl-CoA + electron transfer flavoprotein | - |
Rattus norvegicus | 2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein | - |
r | |
1.3.8.5 | isobutyryl-CoA + acceptor | - |
Rattus norvegicus | methacrylyl-CoA + reduced acceptor | - |
? | |
1.3.8.5 | isovaleryl-CoA + phenazine methosulfate | - |
Rattus norvegicus | ? | - |
r | |
1.3.8.7 | acyl-CoA + acceptor | electron acceptor: phenazine methosulfate | Rattus norvegicus | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | acyl-CoA + acceptor | specific for chain lengths C6-C12 | Rattus norvegicus | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | acyl-CoA + acceptor | intermediary electron acceptor: 2,6-dichloroindophenol | Rattus norvegicus | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | acyl-CoA + acceptor | final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue | Rattus norvegicus | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | decanoyl-CoA + acceptor | 72% of activity with hexanoyl-CoA | Rattus norvegicus | 2-decenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | decanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol | - |
Rattus norvegicus | 2-trans-decenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | heptadecan-2-onyl-dethio-CoA + phenazine methosulfate + 2,6-dichloroindophenol | - |
Rattus norvegicus | ? | - |
? | |
1.3.8.7 | hexadecanoyl-CoA + acceptor | 55% of activity with hexanoyl-CoA | Rattus norvegicus | 2-hexadecenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | hexanoyl-CoA + acceptor | best substrate | Rattus norvegicus | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | hexanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol | - |
Rattus norvegicus | 2-trans-hexenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | lauroyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol | - |
Rattus norvegicus | 2-trans-dodecenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol | 82% of activity with hexanoyl-CoA | Rattus norvegicus | 2-octenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | n-tetradecanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol | best substrate for long-chain acyl-CoA dehydrogenase | Rattus norvegicus | 2-tetradecenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | pentanoyl-CoA + acceptor | 68% of activity with hexanoyl-CoA | Rattus norvegicus | 2-pentenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.8 | decanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E)-2-decenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | dodecanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E)-2-dodecenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | eicosanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | trans-2-eicosenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | octanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-octenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | palmitoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-hexadecenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | stearoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-octadecenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | tetradecanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-tetradecenoyl-CoA + reduced phenazine methosulfate | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.8.4 | FAD | - |
Rattus norvegicus | |
1.3.8.5 | FAD | - |
Rattus norvegicus | |
1.3.8.7 | FAD | FAD is readily lost upon purification | Rattus norvegicus | |
1.3.8.8 | FAD | - |
Rattus norvegicus |