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Literature summary extracted from

  • Ikeda, Y.; Tanaka, K.
    Purification and characterization of 2-methyl-branched chain acyl coenzyme a dehydrogenase, an enzyme involved in the isoleucine and valine metabolism, from rat liver mitochondria (1983), J. Biol. Chem., 258, 9477-9487.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.8.5 2-butenoyl-CoA 0.1 mM, 53% inhibition Rattus norvegicus
1.3.8.5 Ag+
-
Rattus norvegicus
1.3.8.5 butyryl-CoA 0.1 mM, 50% inhibition Rattus norvegicus
1.3.8.5 Cu2+
-
Rattus norvegicus
1.3.8.5 hexanoyl-CoA 19% inhibition Rattus norvegicus
1.3.8.5 Hg2+
-
Rattus norvegicus
1.3.8.5 Methylmercury iodide
-
Rattus norvegicus
1.3.8.5 N-ethylmaleimide
-
Rattus norvegicus
1.3.8.5 p-hydroxymercuribenzoate
-
Rattus norvegicus
1.3.8.5 pentanoyl-CoA 0.1 mM, 50% inhibition Rattus norvegicus
1.3.8.5 tiglyl-CoA more than 95% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.5 0.02
-
2-methylbutyryl-CoA
-
Rattus norvegicus
1.3.8.5 0.089
-
isobutyryl-CoA
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.8.5 mitochondrion
-
Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.5 41500
-
4 * 41500, SDS-PAGE Rattus norvegicus
1.3.8.5 170000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.5 2-methylbutanoyl-CoA + electron transfer flavoprotein Rattus norvegicus
-
2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.5 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.5
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.8.5 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.5 2.345
-
-
Rattus norvegicus
1.3.8.5 2.476
-
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.5 (R)-2-methylbutyryl-CoA + acceptor
-
Rattus norvegicus 2-ethylprop-2-enoyl-CoA + reduced acceptor
-
?
1.3.8.5 (S)-2-methylbutanoyl-CoA + acceptor acceptors: electron transfer flavoprotein, phenazine methosulfate, meldola's blue Rattus norvegicus 2-methylbut-2-enoyl-CoA + reduced acceptor
-
r
1.3.8.5 2-methylbutanoyl-CoA + electron transfer flavoprotein
-
Rattus norvegicus 2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein
-
r
1.3.8.5 butyryl-CoA + acceptor 5% of activity with 2-methylbutyryl-CoA Rattus norvegicus 2-butenoyl-CoA + reduced acceptor
-
?
1.3.8.5 isobutyryl-CoA + acceptor
-
Rattus norvegicus methacrylyl-CoA + reduced acceptor
-
?

Subunits

EC Number Subunits Comment Organism
1.3.8.5 tetramer 4 * 41500, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.8.5 8
-
-
Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.5 FAD 0.5 mol/subunit Rattus norvegicus