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Literature summary extracted from

  • Dommes, V.; Kunau, W.H.
    Purification and properties of acyl coenzyme A dehydrogenases from bovine liver. Formation of 2-trans,4-cis-decadienoyl coenzyme A (1984), J. Biol. Chem., 259, 1789-1797.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.1 0.009
-
 butyryl-CoA
-
 Bos taurus
1.3.8.1 0.02
-
 hexanoyl-CoA
-
 Bos taurus
1.3.8.1 0.032
-
 octanoyl-CoA
-
 Bos taurus
1.3.8.7 0.002
-
 all-cis-4,7,10,13-hexadecatetraenoyl-CoA
-
 Bos taurus
1.3.8.7 0.0021
-
 6-cis-decenoyl-CoA
-
 Bos taurus
1.3.8.7 0.0023
-
 4-cis,7-cis-decadienoyl-CoA
-
 Bos taurus
1.3.8.7 0.0029
-
 decanoyl-CoA
-
 Bos taurus
1.3.8.7 0.0029
-
 4-decynoyl-CoA
-
 Bos taurus
1.3.8.7 0.003
-
 5-cis-decenoyl-CoA
-
 Bos taurus
1.3.8.7 0.0032
-
 octanoyl-CoA
-
 Bos taurus
1.3.8.7 0.004
-
 hexanoyl-CoA
-
 Bos taurus
1.3.8.7 0.004
-
 4-cis-decenoyl-CoA
-
 Bos taurus
1.3.8.7 0.0043
-
 dodecanoyl-CoA
-
 Bos taurus
1.3.8.7 0.005
-
 hexanoyl-CoA
-
 Bos taurus
1.3.8.7 0.0057
-
 tetradecanoyl-CoA
-
 Bos taurus
1.3.8.7 0.0084
-
 butanoyl-CoA
-
 Bos taurus
1.3.8.8 0.13
-
 decanoyl-CoA
-
 Bos taurus
1.3.8.8 0.2
-
 dodecanoyl-CoA
-
 Bos taurus
1.3.8.8 0.2
-
 5-cis-decenoyl-CoA
-
 Bos taurus
1.3.8.8 0.23
-
 octanoyl-CoA
-
 Bos taurus
1.3.8.8 0.24
-
 cis,cis,cis,cis-4,7,10,13-hexadecatetraenoyl-CoA
-
 Bos taurus
1.3.8.8 0.25
-
 4-cis-decenoyl-CoA
-
 Bos taurus
1.3.8.8 0.29
-
 stearoyl-CoA
-
 Bos taurus
1.3.8.8 0.32
-
 4-cis,7-cis-decadienoyl-CoA
-
 Bos taurus
1.3.8.8 0.32
-
 6-cis-decenoyl-CoA
-
 Bos taurus
1.3.8.8 0.38
-
 tetradecanoyl-CoA
-
 Bos taurus
1.3.8.8 0.55
-
 hexanoyl-CoA
-
 Bos taurus
1.3.8.8 0.78
-
 palmitoyl-CoA
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.8.8 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.1 40300
-
 4 * 40300, SDS-PAGE Bos taurus
1.3.8.1 188000
-
 gel filtration Bos taurus
1.3.8.7 43300
-
 4 * 43300, SDS-PAGE Bos taurus
1.3.8.7 205000
-
 native gel electrophoresis in polyacrylamide gradient Bos taurus
1.3.8.8 172000
-
 gel electrophoresis Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.1 Bos taurus
-
-
-
1.3.8.7 Bos taurus
-
-
-
1.3.8.8 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.1
-
 Bos taurus
1.3.8.7
-
 Bos taurus
1.3.8.8 40-70% ammonium sulfate precipitation, Sephadex G-150, DEAE-cellulose, preparative electrophoresis Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.8.1 liver
-
 Bos taurus
-
1.3.8.7 liver
-
 Bos taurus
-
1.3.8.8 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.1 1.42
-
-
 Bos taurus
1.3.8.1 17.9
-
-
 Bos taurus
1.3.8.8 32.5
-
 purified enzyme, decanoyl-CoA Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.1 butyryl-CoA + electron acceptor intermediate electron acceptors phenazine methosulfate or Meldola' s Blue (i.e. 8-dimethylamino-2,3-benzophenoxazinium chloride) Bos taurus 2-butenoyl-CoA + reduced acceptor
-
 ?
1.3.8.1 butyryl-CoA + electron acceptor terminal electron acceptor iodonitrotetrazolium chloride Bos taurus 2-butenoyl-CoA + reduced acceptor
-
 ?
1.3.8.1 hexanoyl-CoA + phenazine methosulfate + dichloroindophenol 52% of activity with butyryl-CoA Bos taurus 2-hexenoyl-CoA + reduced acceptor
-
 ?
1.3.8.1 octanoyl-CoA + Meldola's Blue + iodonitrotetrazolium chloride 14.4% of activity with butyryl-CoA Bos taurus 2-octenoyl-CoA + reduced acceptor
-
 ?
1.3.8.7 4-cis,7-cis-decadienoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase Bos taurus 2-trans,4-cis,7cis-decatrienoyl-CoA + reduced acceptor
-
 ?
1.3.8.7 4-cis-decenoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase Bos taurus 2-trans,4-cis-decadienoyl-CoA + reduced acceptor
-
 ?
1.3.8.7 4-decynoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase Bos taurus trans-2-ene-4-decynoyl-CoA + reduced acceptor
-
 ?
1.3.8.7 5-cis-decenoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase Bos taurus 2-trans,5-cis-decadienoyl-CoA + reduced acceptor
-
 ?
1.3.8.7 6-cis-decenoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase Bos taurus 2-trans,6-cis-decadienoyl-CoA + reduced acceptor
-
 ?
1.3.8.7 acyl-CoA + acceptor 2-p-iodophenyl-3-p-nitrophenyl-5-phenyl-2H-tetrazolium chloride as final electron acceptor Bos taurus 2,3-dehydroacyl-CoA + reduced acceptor
-
 ?
1.3.8.7 all-cis-4,7,10,13-hexadecatetraenoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase Bos taurus 2-trans-all-cis-4,7,10,13-hexadecapentaenoyl-CoA + reduced acceptor
-
 ?
1.3.8.7 butanoyl-CoA + acceptor
-
 Bos taurus crotonyl-CoA + reduced acceptor
-
 ?
1.3.8.7 decanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol electron acceptor: Meldola's Blue and iodonitrotetrazoliumchloride, general and long-chain acyl-CoA dehydrogenase Bos taurus 2-trans-decenoyl-CoA + reduced acceptor
-
 ?
1.3.8.7 dodecanoyl-CoA + tetrazolium salt
-
 Bos taurus trans-dodec-2-enoyl-CoA + reduced tetrazolium salt
-
 r
1.3.8.7 hexadecanoyl-CoA + Meldola's Blue + iodonitrotetrazoliumchloride cis-double bond has almost no effect on velocity of general acyl-CoA dehydrogenase Bos taurus 2-trans-hexadecenoyl-CoA + reduced acceptor
-
 ?
1.3.8.7 hexanoyl-CoA + tetrazolium salt
-
 Bos taurus trans-hex-2-enoyl-CoA + reduced tetrazolium salt
-
 r
1.3.8.7 tetradecanoyl-CoA + tetrazolium salt
-
 Bos taurus tetradecenoyl-CoA + reduced tetrazolium salt
-
 r
1.3.8.8 4-cis-7-cis-decadienoyl-CoA + Meldola's Blue
-
 Bos taurus 2-trans-4,7-cis,cis-decatrienoyl-CoA + reduced meldola blue
-
 ?
1.3.8.8 4-cis-decenoyl-CoA + Meldola's Blue
-
 Bos taurus 2-trans-4-cis-decadienoyl-CoA + reduced meldola blue
-
 ?
1.3.8.8 5-cis-decenoyl-CoA + Meldola's Blue
-
 Bos taurus 2-trans-5-cis-decadienoyl-CoA + reduced meldola blue
-
 ?
1.3.8.8 6-cis-decenoyl-CoA + Meldola's Blue
-
 Bos taurus 2-trans-6-cis-decadienoyl-CoA + reduced meldola blue
-
 ?
1.3.8.8 cis,cis,cis,cis-4,7,10,13-hexadecatetraenoyl-CoA + Meldola's Blue
-
 Bos taurus 2-trans-4,7,10,13-cis,cis,cis,cis-hexadecapentaenoyl-CoA + reduced meldola blue
-
 ?
1.3.8.8 decanoyl-CoA + Meldola's Blue
-
 Bos taurus 2-decenoyl-CoA + reduced meldola blue trans-isomer ?
1.3.8.8 dodecanoyl-CoA + Meldola's Blue
-
 Bos taurus 2-dodecenoyl-CoA + reduced meldola blue trans-isomer ?
1.3.8.8 hexanoyl-CoA + Meldola's Blue
-
 Bos taurus (2E)-2-hexenoyl-CoA + reduced meldola blue trans-isomer ?
1.3.8.8 octanoyl-CoA + Meldola's Blue
-
 Bos taurus (2E)-2-octenoyl-CoA + reduced meldola blue trans-isomer ?
1.3.8.8 palmitoyl-CoA + Meldola's Blue
-
 Bos taurus 2-hexadecenoyl-CoA + reduced meldola blue trans-isomer ?
1.3.8.8 stearoyl-CoA + Meldola's Blue
-
 Bos taurus 2-octadecenoyl-CoA + reduced meldola blue trans-isomer ?
1.3.8.8 tetradecanoyl-CoA + Meldola's Blue
-
 Bos taurus 2-tetradecenoyl-CoA + reduced meldola blue trans-isomer ?

Subunits

EC Number Subunits Comment Organism
1.3.8.1 tetramer 4 * 40300, SDS-PAGE Bos taurus
1.3.8.7 tetramer 4 * 43300, SDS-PAGE Bos taurus
1.3.8.8 tetramer 4 * 38000-45200, SDS-PAGE Bos taurus