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Literature summary extracted from

  • Haertel, U.; Eckel, E.; Koch, J.; Fuchs, G.; Linder, D.; Buckel, W.
    Purification of glutaryl-CoA dehydrogenase from Pseudomonas sp., an enzyme involved in the anaerobic degradation of benzoate (1993), Arch. Microbiol., 159, 174-181.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.4.5 glutaryl-CoA competitive to glutaconyl-CoA Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.6 0.003 0.005 glutaryl-CoA
-
 Pseudomonas sp.
1.3.8.6 0.006 0.011 glutaconyl-CoA
-
 Pseudomonas sp.
7.2.4.5 3 5 glutaryl-CoA 28°C, pH 7.5 Pseudomonas sp.
7.2.4.5 6 11 glutaconyl-CoA 28°C, pH 7.5 Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.4.5 soluble
-
 Pseudomonas sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.6 41000
-
 4 * 41000, SDS-PAGE Pseudomonas sp.
1.3.8.6 170000
-
 gel filtration Pseudomonas sp.
7.2.4.5 41000
-
 4 * 41000, SDS-PAGE Pseudomonas sp.
7.2.4.5 170000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.4.5 glutaconyl-CoA + Na+[side 1] Pseudomonas sp.
-
 but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.6 Pseudomonas sp.
-
 strains KB 740, K 172
-
7.2.4.5 Pseudomonas sp.
-
 enzyme also decarboxylates glutaryl-CoA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.6
-
 Pseudomonas sp.
7.2.4.5
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.3.8.6 -20°C, 20 mM potassium phosphate, 150 mM NaCl, pH 6.4, several months without loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.6 glutaconyl-CoA + acceptor
-
 Pseudomonas sp. crotonyl-CoA + CO2 + reduced acceptor
-
 r
1.3.8.6 glutaryl-CoA + electron-transfer flavoprotein
-
 Pseudomonas sp. crotonyl-CoA + CO2 + reduced electron-transfer flavoprotein
-
 r
7.2.4.5 4-carboxybut-2-enoyl-CoA + Na+[side 1]
-
 Pseudomonas sp. but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 glutaconyl-CoA + Na+[side 1]
-
 Pseudomonas sp. but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
7.2.4.5 glutaryl-CoA + Na+[side 1]
-
 Pseudomonas sp. butyryl-CoA + CO2 + Na+[side 2]
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.8.6 tetramer 4 * 41000, SDS-PAGE Pseudomonas sp.
7.2.4.5 tetramer 4 * 41000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.8.6 28
-
 assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.8.6 7.5 8
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.6 FAD 1.1 mol per mol of subunit Pseudomonas sp.
7.2.4.5 FAD 1.1 mol of FAD per subunit, Km: 0.00025 mM Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.2.4.5 0.0074
-
 glutaryl-CoA
-
 Pseudomonas sp.