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Literature summary extracted from
- Purification and some properties of glutamate synthase from Gluconobacter subodydans on glutamate as a nitrogen source (1983), J. Ferment. Technol., 61, 179-184.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.4.1.13 | alpha-ketoglutarate stabilizes | Gluconobacter oxydans |
| 1.4.1.13 | EDTA, stabilizes | Gluconobacter oxydans |
| 1.4.1.13 | glutamine stabilizes | Gluconobacter oxydans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.13 | alpha-aminobutyrate | - |
Gluconobacter oxydans |  |
| 1.4.1.13 | D-glutamate | - |
Gluconobacter oxydans | |
| 1.4.1.13 | D-glutamine | - |
Gluconobacter oxydans | |
| 1.4.1.13 | DL-methionine DL-sulfoximine | potent inhibitor | Gluconobacter oxydans | |
| 1.4.1.13 | DL-Theanine | - |
Gluconobacter oxydans | |
| 1.4.1.13 | hydroxylamine | - |
Gluconobacter oxydans | |
| 1.4.1.13 | L-alanine | - |
Gluconobacter oxydans | |
| 1.4.1.13 | L-aspartate | - |
Gluconobacter oxydans | |
| 1.4.1.13 | L-histidine | - |
Gluconobacter oxydans | |
| 1.4.1.13 | L-homoserine | - |
Gluconobacter oxydans | |
| 1.4.1.13 | L-leucine | - |
Gluconobacter oxydans | |
| 1.4.1.13 | L-methionine | - |
Gluconobacter oxydans | |
| 1.4.1.13 | lysine | - |
Gluconobacter oxydans | |
| 1.4.1.13 | NADH | partially inhibits | Gluconobacter oxydans | |
| 1.4.1.13 | NADP+ | potent inhibitor | Gluconobacter oxydans | |
| 1.4.1.13 | oxaloacetate | - |
Gluconobacter oxydans | |
| 1.4.1.13 | putrescine | - |
Gluconobacter oxydans | |
| 1.4.1.13 | pyruvate | - |
Gluconobacter oxydans | |
| 1.4.1.13 | valine | - |
Gluconobacter oxydans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.13 | 0.0086 | - |
alpha-ketoglutarate | - |
Gluconobacter oxydans | |
| 1.4.1.13 | 0.014 | - |
NADPH | - |
Gluconobacter oxydans | |
| 1.4.1.13 | 0.59 | - |
L-glutamine | - |
Gluconobacter oxydans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.13 | 500000 | - |
gel filtration | Gluconobacter oxydans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.1.13 | Gluconobacter oxydans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.1.13 | using ammonium sulfate fractionation, coulmn chromatography on DEAE-cellulose, hydroxyapatite, Sepharose 6B and Sephadex G-200 | Gluconobacter oxydans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.13 | 5.72 | - |
- |
Gluconobacter oxydans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.13 | L-glutamine + 2-oxoglutarate + NADPH + H+ | highly specific | Gluconobacter oxydans | L-glutamate + NADP+ | - |
? | |
| 1.4.1.13 | additional information | amino acids, amines and ammonium chloride do not substitute for L-glutamine, other alpha-keto acids including oxalacetate, pyruvate, glyoxylate and alpha-ketobutyrate do not support the activity | Gluconobacter oxydans | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.13 | 45 | - |
the enzyme retains its full activity after heating for 10 min in 0.1 M potassium phosphate buffer, pH 6.8 | Gluconobacter oxydans |
| 1.4.1.13 | 55 | - |
10 min, in 0.1 M potassium phosphate buffer, pH 6.8, 70-80% loss of activity | Gluconobacter oxydans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.13 | 8.5 | - |
- |
Gluconobacter oxydans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.13 | 8 | 9 | - |
Gluconobacter oxydans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.13 | NADPH | - |
Gluconobacter oxydans | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.13 | 0.02 | - |
DL-methionine DL-sulfoximine | competitive inhibitor with respect to alpha-keto-glutarate | Gluconobacter oxydans | |
| 1.4.1.13 | 0.07 | - |
NADP | competitive inhibition with NADPH | Gluconobacter oxydans | |
| 1.4.1.13 | 0.12 | - |
NADP | mixed-type inhibition with alpha-ketoglutarate | Gluconobacter oxydans | |
| 1.4.1.13 | 0.14 | - |
DL-methionine DL-sulfoximine | competitive inhibitor with respect to glutamine | Gluconobacter oxydans | |
| 1.4.1.13 | 0.3 | - |
NADP | uncompetitive inhibition with respect to glutamine | Gluconobacter oxydans | |
| 1.4.1.13 | 0.48 | - |
methionine | competitive inhibitor with respect to alpha-keto-glutarate | Gluconobacter oxydans | |
| 1.4.1.13 | 0.76 | - |
DL-methionine DL-sulfoximine | noncompetitive inhibitor with respect to NADPH | Gluconobacter oxydans | |
| 1.4.1.13 | 3 | - |
methionine | competitive inhibitor with respect to glutamine | Gluconobacter oxydans | |
| 1.4.1.13 | 5.5 | - |
methionine | noncompetitive inhibitor with respect to NADPH | Gluconobacter oxydans | |
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