EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.8 | L-valine | VDH synthesis induced by L-valine, but severely repressed by ammonia | Kitasatospora aureofaciens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.8 | iodoacetamide | VDH2: 1 mM, 50% loss of activity | Kitasatospora aureofaciens | |
1.4.1.8 | Mn2+ | VDH2: 1 mM, 60% loss of activity | Kitasatospora aureofaciens | |
1.4.1.8 | p-hydroxymercuribenzoate | VDH2: 0.01 mM, complete inhibition | Kitasatospora aureofaciens | |
1.4.1.23 | 4-hydroxymercuribenzoate | 0.01 mM, 100% inhibition | Kitasatospora aureofaciens | |
1.4.1.23 | iodoacetamide | 1 mM, 50% inhibition | Kitasatospora aureofaciens | |
1.4.1.23 | Mn2+ | 1 mM, 60% inhibition | Kitasatospora aureofaciens | |
1.4.1.23 | additional information | activity is not significantly affected by 1 mM ATP, ADP, AMP, Mg2+, Ca2+, Cu2+, Zn2+, and metabolites of the citric acid cycle | Kitasatospora aureofaciens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.8 | 0.46 | - |
2-oxoisovalerate | VDH2 | Kitasatospora aureofaciens | |
1.4.1.8 | 0.75 | - |
L-valine | VDH2 | Kitasatospora aureofaciens | |
1.4.1.8 | 1.06 | - |
2-oxo-3-methylvalerate | VDH2 | Kitasatospora aureofaciens | |
1.4.1.8 | 1.2 | - |
2-oxoisocaproate | VDH2 | Kitasatospora aureofaciens | |
1.4.1.8 | 1.66 | - |
L-norvaline | VDH2 | Kitasatospora aureofaciens | |
1.4.1.8 | 1.72 | - |
L-isoleucine | VDH2 | Kitasatospora aureofaciens | |
1.4.1.8 | 1.85 | - |
L-leucine | VDH2 | Kitasatospora aureofaciens | |
1.4.1.8 | 4.35 | - |
L-2-aminobutyrate | VDH2 | Kitasatospora aureofaciens | |
1.4.1.8 | 17.2 | - |
pyruvate | VDH2 | Kitasatospora aureofaciens | |
1.4.1.8 | 22.5 | - |
L-norleucine | VDH2 | Kitasatospora aureofaciens | |
1.4.1.8 | 117.6 | - |
L-methionine | VDH2 | Kitasatospora aureofaciens | |
1.4.1.8 | 333.3 | - |
L-alanine | VDH2 | Kitasatospora aureofaciens | |
1.4.1.23 | 0.42 | - |
2-Oxoisohexanoate | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.4.1.23 | 0.46 | - |
2-oxoisovalerate | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.4.1.23 | 0.75 | - |
L-valine | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.4.1.23 | 1.06 | - |
3-methyl-2-oxopentanoate | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.4.1.23 | 1.2 | - |
2-oxobutanoate | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.4.1.23 | 1.66 | - |
L-norvaline | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.4.1.23 | 1.72 | - |
L-isoleucine | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.4.1.23 | 1.85 | - |
L-leucine | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.4.1.23 | 4.35 | - |
L-2-aminobutanoate | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.4.1.23 | 17.2 | - |
pyruvate | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.4.1.23 | 22.5 | - |
L-norleucine | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.4.1.23 | 333 | - |
L-alanine | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.8 | 41000 | - |
6 * 41000, VDH2, SDS-PAGE | Kitasatospora aureofaciens |
1.4.1.8 | 240000 | - |
VDH2, gel filtration | Kitasatospora aureofaciens |
1.4.1.23 | 41000 | - |
6 * 41000, SDS-PAGE | Kitasatospora aureofaciens |
1.4.1.23 | 240000 | - |
gel filtration | Kitasatospora aureofaciens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.8 | L-valine + NAD(P)+ + H2O | Kitasatospora aureofaciens | VDH is required for utilization of branched chain amino acids, the catabolism appears to be an alternative source of n-butyrate, 2-methylmalonate, and propionate needed for biosynthesis of macrolide and polyether antibiotics | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | Kitasatospora aureofaciens | first step in valine catabolism | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | Kitasatospora aureofaciens BS-5 | VDH is required for utilization of branched chain amino acids, the catabolism appears to be an alternative source of n-butyrate, 2-methylmalonate, and propionate needed for biosynthesis of macrolide and polyether antibiotics | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | Kitasatospora aureofaciens BS-5 | first step in valine catabolism | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.8 | Kitasatospora aureofaciens | - |
- |
- |
1.4.1.8 | Kitasatospora aureofaciens | - |
two isoenzymes: VDH1 and VDH2 | - |
1.4.1.8 | Kitasatospora aureofaciens BS-5 | - |
- |
- |
1.4.1.23 | Kitasatospora aureofaciens | - |
- |
- |
1.4.1.23 | Kitasatospora aureofaciens BS-5 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.8 | VDH2, 52.6fold purification | Kitasatospora aureofaciens |
1.4.1.23 | - |
Kitasatospora aureofaciens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.8 | 45.01 | - |
NAD+ | Kitasatospora aureofaciens |
1.4.1.8 | 64.31 | - |
oxidative deamination | Kitasatospora aureofaciens |
1.4.1.8 | 366.5 | - |
reductive amination | Kitasatospora aureofaciens |
EC Number | Storage Stability | Organism |
---|---|---|
1.4.1.8 | -80°C, 100 mM Tris-HCl buffer, pH 7.4, 20% v/v glycerol, 2 months, stable | Kitasatospora aureofaciens |
1.4.1.23 | -80°C, presence of 20% glycerol, stable for at least 2 months | Kitasatospora aureofaciens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.8 | L-2-aminobutyrate + H2O + NAD+ | VDH2: 125% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxobutyrate + NADH + NH3 | VDH2: reductive amination, 116% of the activity with 2-oxoisovalerate | r | |
1.4.1.8 | L-2-aminobutyrate + H2O + NAD+ | VDH2: 125% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxobutyrate + NADH + NH3 | 2-oxobutyrate is identical with alpha-ketobutyrate | r | |
1.4.1.8 | L-2-aminobutyrate + H2O + NAD+ | L-2-aminobutyrate is identical with L-alpha-aminobutyrate | Kitasatospora aureofaciens | 2-oxobutyrate + NADH + NH3 | VDH2: reductive amination, 116% of the activity with 2-oxoisovalerate | r | |
1.4.1.8 | L-2-aminobutyrate + H2O + NAD+ | L-2-aminobutyrate is identical with L-alpha-aminobutyrate | Kitasatospora aureofaciens | 2-oxobutyrate + NADH + NH3 | 2-oxobutyrate is identical with alpha-ketobutyrate | r | |
1.4.1.8 | L-alanine + H2O + NAD+ | VDH2: 4.5% of the activity with L-valine | Kitasatospora aureofaciens | pyruvate + NH3 + NADH | pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate | r | |
1.4.1.8 | L-alanine + H2O + NAD+ | VDH2: 4.5% of the activity with L-valine | Kitasatospora aureofaciens | pyruvate + NH3 + NADH | VDH2: reductive amination, 20.8% of the activity with 2-oxoisovalerate | r | |
1.4.1.8 | L-isoleucine + H2O + NAD+ | VDH2: 34% of the activity with L-valine | Kitasatospora aureofaciens | 3-methyl-2-oxopentanoate + NADH + NH3 | 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate | r | |
1.4.1.8 | L-isoleucine + H2O + NAD+ | VDH2: 34% of the activity with L-valine | Kitasatospora aureofaciens | 3-methyl-2-oxopentanoate + NADH + NH3 | VDH2: reductive amination, 24.5% of the activity with 2-oxoisovalerate | r | |
1.4.1.8 | L-leucine + H2O + NAD+ | VDH2: 52.8% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxoisocaproate + NADH + NH3 | 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate | r | |
1.4.1.8 | L-leucine + H2O + NAD+ | VDH2: 52.8% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxoisocaproate + NADH + NH3 | VDH2: reductive amination, 26.5% of the activity with 2-oxoisovalerate | r | |
1.4.1.8 | L-methionine + H2O + NAD+ | VDH2: 2% of the activity with L-valine | Kitasatospora aureofaciens | 4-(methylthio)-2-oxobutanoic acid + NH3 + NADH | - |
r | |
1.4.1.8 | L-norleucine + H2O + NAD+ | VDH2: 18.5% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxocaproate + NADH + NH3 | - |
r | |
1.4.1.8 | L-norvaline + H2O + NAD+ | VDH2: 100% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxovalerate + NH3 + NADH | - |
r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Kitasatospora aureofaciens | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Kitasatospora aureofaciens | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Kitasatospora aureofaciens | 2-oxoisovalerate + NH3 + NAD(P)H | reductive amination: preferred substrate | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | VDH is required for utilization of branched chain amino acids, the catabolism appears to be an alternative source of n-butyrate, 2-methylmalonate, and propionate needed for biosynthesis of macrolide and polyether antibiotics | Kitasatospora aureofaciens | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | first step in valine catabolism | Kitasatospora aureofaciens | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Kitasatospora aureofaciens BS-5 | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Kitasatospora aureofaciens BS-5 | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Kitasatospora aureofaciens BS-5 | 2-oxoisovalerate + NH3 + NAD(P)H | reductive amination: preferred substrate | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | VDH is required for utilization of branched chain amino acids, the catabolism appears to be an alternative source of n-butyrate, 2-methylmalonate, and propionate needed for biosynthesis of macrolide and polyether antibiotics | Kitasatospora aureofaciens BS-5 | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | first step in valine catabolism | Kitasatospora aureofaciens BS-5 | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? | |
1.4.1.23 | 2-oxobutanoate + NH3 + NADH + H+ | 116% of the activity with 2-oxoisovalerate | Kitasatospora aureofaciens | L-2-aminobutanoate + H2O + NAD+ | - |
r | |
1.4.1.23 | 2-oxoisohexanoate + NH3 + NADH + H+ | 26.5% of the activity with 2-oxoisovalerate | Kitasatospora aureofaciens | L-leucine + H2O + NAD+ | - |
r | |
1.4.1.23 | 2-oxoisovalerate + NH3 + NADH + H+ | - |
Kitasatospora aureofaciens | L-valine + H2O + NAD+ | - |
r | |
1.4.1.23 | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | 24.5% of the activity with 2-oxoisovalerate | Kitasatospora aureofaciens | L-isoleucine + H2O + NAD+ | - |
r | |
1.4.1.23 | L-2-aminobutanoate + H2O + NAD+ | 125% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-alanine + H2O + NAD+ | 4.5% of the activity with L-valine | Kitasatospora aureofaciens | pyruvate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-isoleucine + H2O + NAD+ | 34% of the activity with L-valine | Kitasatospora aureofaciens | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-leucine + H2O + NAD+ | 52.8% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxoisohexanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-norleucine + H2O + NAD+ | 18.5% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxohexanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-norvaline + H2O + NAD+ | 100% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxovalerate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-valine + H2O + NAD+ | - |
Kitasatospora aureofaciens | 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | additional information | no substrates: D-valine, glycine, L-threonine, L-serine, L-cysteine, L-glutamate, L-aspartate, L-asparagine, L-arginine, L-histidine, L-tryptophan, and DL-phenylalanine, and 2-ketoglutarate and oxalacetate | Kitasatospora aureofaciens | ? | - |
? | |
1.4.1.23 | pyruvate + H2O + NAD+ | 20.8% of the activity with 2-oxoisovalerate | Kitasatospora aureofaciens | L-alanine + NH3 + NADH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.8 | hexamer | 6 * 41000, VDH2, SDS-PAGE | Kitasatospora aureofaciens |
1.4.1.23 | hexamer | 6 * 41000, SDS-PAGE | Kitasatospora aureofaciens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.1.23 | VDH2 | - |
Kitasatospora aureofaciens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.8 | 65 | - |
- |
Kitasatospora aureofaciens |
1.4.1.23 | 65 | - |
both reduxtive amination and oxidative deamination | Kitasatospora aureofaciens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.8 | 9 | - |
VDH2: reductive amination of 2-oxoisovalerate, 0.3 M Tris-HCl buffer | Kitasatospora aureofaciens |
1.4.1.8 | 10.4 | - |
VDH2: oxidative deamination of L-valine, 0.3 M glycine-KCl-KOH buffer | Kitasatospora aureofaciens |
1.4.1.23 | 9 | - |
reductive amination | Kitasatospora aureofaciens |
1.4.1.23 | 10.4 | - |
oxidative deamination | Kitasatospora aureofaciens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.8 | 1,N6-etheno-NAD+ | VDH2: 62.4% of the activity with NAD+ | Kitasatospora aureofaciens | |
1.4.1.8 | 3-pyridinealdehyde-NAD+ | VDH2: 37.5% of the activity with NAD+ | Kitasatospora aureofaciens | |
1.4.1.8 | Alpha-NAD+ | VDH2: 5% of the activity with NAD+, VDH1: no activity | Kitasatospora aureofaciens | |
1.4.1.8 | deamino-NAD+ | VDH2: 375.5% of the activity with NAD+ | Kitasatospora aureofaciens | |
1.4.1.8 | additional information | VDH2: not with 3-acetylpyridine-NAD+, compared to VDH1 with 100% of the activity with NAD+ | Kitasatospora aureofaciens | |
1.4.1.8 | additional information | not with NADP+ and NADPH | Kitasatospora aureofaciens | |
1.4.1.8 | NAD+ | NAD+ required, no use of NADP+ | Kitasatospora aureofaciens | |
1.4.1.8 | NAD+ | natural cofactor for oxidative deamination | Kitasatospora aureofaciens | |
1.4.1.8 | NADH | NADH could not be replaced with NADPH for the reductive amination | Kitasatospora aureofaciens | |
1.4.1.8 | nicotinamide guanine dinucleotide | VDH2: 80% of the activity with NAD+ | Kitasatospora aureofaciens | |
1.4.1.23 | 1,N6-etheno-NAD+ | 62% of the activity with NAD+ | Kitasatospora aureofaciens | |
1.4.1.23 | deamino-NAD+ | 373% of the activity with NAD+ | Kitasatospora aureofaciens | |
1.4.1.23 | additional information | no cofactor: 3-acetylpyridine-NAD+, thionicotinamide-NAD+, alpha-NAD+, 3-pyridinaldehyde-NAD+, deamido-NAD+ | Kitasatospora aureofaciens | |
1.4.1.23 | NAD+ | - |
Kitasatospora aureofaciens | |
1.4.1.23 | NADH | - |
Kitasatospora aureofaciens | |
1.4.1.23 | nicotinamide guanine dinucleotide | 80% of the activity with NAD+ | Kitasatospora aureofaciens |