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Literature summary extracted from

  • Yurimoto, H.; Hasegawa, T.; Sakai, Y.; Kato, N.
    Characterization and high-level production of D-amino acid oxidase in Candida boidinii (2001), Biosci. Biotechnol. Biochem., 65, 627-633.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 overexpression in Candida boidinii strain aod1 DELTA [Candida] boidinii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 Ag+ 1 mM [Candida] boidinii
1.4.3.3 Hg2+ 1 mM [Candida] boidinii
1.4.3.3 iodoacetamide 1 mM [Candida] boidinii
1.4.3.3 p-chloromercuribenzoate 0.1 mM [Candida] boidinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 3.95
-
 D-valine
-
 [Candida] boidinii
1.4.3.3 4.28
-
 D-alanine
-
 [Candida] boidinii
1.4.3.3 10.9
-
 D-leucine
-
 [Candida] boidinii
1.4.3.3 27.4
-
 D-methionine
-
 [Candida] boidinii
1.4.3.3 33.7
-
 D-serine
-
 [Candida] boidinii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.3.3 peroxisome
-
 [Candida] boidinii 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.3 41000
-
 1 * 41000, SDS-PAGE [Candida] boidinii
1.4.3.3 43000
-
 gel filtration [Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2 [Candida] boidinii
-
 pyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-asparagine + H2O + O2 [Candida] boidinii
-
 2-oxosuccinamic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-leucine + H2O + O2 [Candida] boidinii
-
 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2 [Candida] boidinii
-
 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2 [Candida] boidinii
-
 phenylpyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-proline + H2O + O2 [Candida] boidinii
-
 2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-serine + H2O + O2 [Candida] boidinii
-
 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-threonine + H2O + O2 [Candida] boidinii
-
 2-oxo-3-hydroxybutyric acid + NH3 + H2O2
-
 ?
1.4.3.3 D-valine + H2O + O2 [Candida] boidinii
-
 alpha-ketoisovaleric acid + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 [Candida] boidinii
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.3 139
-
-
 [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2
-
 [Candida] boidinii pyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-asparagine + H2O + O2
-
 [Candida] boidinii 2-oxosuccinamic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-leucine + H2O + O2
-
 [Candida] boidinii 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2
-
 [Candida] boidinii 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2
-
 [Candida] boidinii phenylpyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-proline + H2O + O2
-
 [Candida] boidinii 2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-serine + H2O + O2
-
 [Candida] boidinii 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-threonine + H2O + O2
-
 [Candida] boidinii 2-oxo-3-hydroxybutyric acid + NH3 + H2O2
-
 ?
1.4.3.3 D-valine + H2O + O2
-
 [Candida] boidinii alpha-ketoisovaleric acid + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.3 monomer 1 * 41000, SDS-PAGE [Candida] boidinii

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD
-
 [Candida] boidinii