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Literature summary extracted from

  • Fahmy, A.S.; Mohamed, S.A.; Girgis, R.B.; Abdel-Ghaffar, F.A.
    Enzymes of DELTA1-pyrroline-5-carboxylate metabolism in the camel tick Hyalomma dromedarii during embryogenesis. Purification and characterization of DELTA1-pyrroline-5-carboxylate dehydrogenases (1997), Comp. Biochem. Physiol. B, 118, 229-237.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.88 4-aminobutyrate both isoforms Hyalomma dromedarii
1.2.1.88 ADP 3 mM, 14% inhibition of isoform A and 16% inhibition of isoform B Hyalomma dromedarii
1.2.1.88 AMP 3 mM, 33% inhibition of isoform A and 11% inhibition of isoform B Hyalomma dromedarii
1.2.1.88 arsenite both isoforms Hyalomma dromedarii
1.2.1.88 ATP inhibits isoform B by 33%, but not isoform A Hyalomma dromedarii
1.2.1.88 Co2+ both isoforms, concentration above 3 mM Hyalomma dromedarii
1.2.1.88 Gly
-
 Hyalomma dromedarii
1.2.1.88 Hg2+ both isoforms Hyalomma dromedarii
1.2.1.88 iodoacetamide both isoforms Hyalomma dromedarii
1.2.1.88 L-Ala
-
 Hyalomma dromedarii
1.2.1.88 L-Glu
-
 Hyalomma dromedarii
1.2.1.88 L-hydroxyproline both isoforms, potent inhibition Hyalomma dromedarii
1.2.1.88 L-Leu
-
 Hyalomma dromedarii
1.2.1.88 L-Ser
-
 Hyalomma dromedarii
1.2.1.88 L-Val
-
 Hyalomma dromedarii
1.2.1.88 Ni2+ both isoforms Hyalomma dromedarii
1.2.1.88 p-hydroxymercuribenzoate both isoforms, potent inhibition Hyalomma dromedarii
1.2.1.88 proline both isoforms Hyalomma dromedarii
1.2.1.88 Zn2+ both isoforms Hyalomma dromedarii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.88 0.133
-
 D-glucose isoform A Hyalomma dromedarii
1.2.1.88 0.2
-
 1-pyrroline-5-carboxylate isoform B, with NADP+ Hyalomma dromedarii
1.2.1.88 0.2
-
 indol-3-acetaldehyde isoform A Hyalomma dromedarii
1.2.1.88 0.25
-
 4-methoxybenzaldehyde isoform A Hyalomma dromedarii
1.2.1.88 0.27
-
 p-nitrobenzaldehyde isoform A Hyalomma dromedarii
1.2.1.88 0.3
-
 1-pyrroline-5-carboxylate isoform B, with NAD+ Hyalomma dromedarii
1.2.1.88 0.3
-
 1-pyrroline-5-carboxylate isoform A, with NADP+ Hyalomma dromedarii
1.2.1.88 0.33
-
 1-pyrroline-5-carboxylate isoform A, with NAD+ Hyalomma dromedarii
1.2.1.88 0.5
-
 benzaldehyde isoform A Hyalomma dromedarii
1.2.1.88 0.5
-
 3-Methoxybenzaldehyde isoform A Hyalomma dromedarii
1.2.1.88 0.62
-
 NAD+ isoform A Hyalomma dromedarii
1.2.1.88 0.76
-
 formaldehyde isoform A Hyalomma dromedarii
1.2.1.88 1
-
 NADP+ isoform A Hyalomma dromedarii
1.2.1.88 1
-
 acetaldehyde isoform A Hyalomma dromedarii
1.2.1.88 1
-
 glyoxylic acid isoform A Hyalomma dromedarii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.88 50000
-
 isoform B, gel filtration Hyalomma dromedarii
1.2.1.88 52000
-
 1 * 52000, isoform B, SDS-PAGE Hyalomma dromedarii
1.2.1.88 59000
-
 1 * 59000, isoform A, SDS-PAGE Hyalomma dromedarii
1.2.1.88 60000
-
 isoform A, gel filtration Hyalomma dromedarii

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.88 Hyalomma dromedarii
-
 two isoforms A and B
-
1.2.1.88 Hyalomma dromedarii
-
 camel tick
-
1.5.1.2 Hyalomma dromedarii
-
 camel
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.88 amino acid composition determination Hyalomma dromedarii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.88 embryo
-
 Hyalomma dromedarii
-
1.2.1.88 larva
-
 Hyalomma dromedarii
-
1.5.1.2 embryo
-
 Hyalomma dromedarii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.88 0.151
-
 embryo, 24 days after oviposition Hyalomma dromedarii
1.2.1.88 0.223
-
 larvae Hyalomma dromedarii
1.2.1.88 9.75
-
 purified enzyme Hyalomma dromedarii
1.5.1.2 additional information
-
 specific activity in different developmental stages of the embryo Hyalomma dromedarii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Hyalomma dromedarii L-glutamate + NADH
-
 ?
1.2.1.88 1-pyrroline-5-carboxylate + NADP+ + H2O
-
 Hyalomma dromedarii L-glutamate + NADPH + H+
-
 r
1.2.1.88 3-methoxybenzaldehyde + NAD+ + H2O as active as 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B Hyalomma dromedarii 3-methoxybenzoate + NADH
-
 ?
1.2.1.88 4-methoxybenzaldehyde + NAD+ + H2O as active as 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B Hyalomma dromedarii 4-methoxybenzoate + NADH + H+
-
 ?
1.2.1.88 4-nitrobenzaldehyde + NAD+ + H2O as active as 1-pyrroline-5-carboxylate with isoform A, only slight activity with isoform B Hyalomma dromedarii 4-nitrobenzoate + NADH + H+
-
 ?
1.2.1.88 acetaldehyde + NAD+ + H2O 41% activity compared to 1-pyrroline-5-carboxylate with isoform A, only slight activity with isoform B Hyalomma dromedarii acetate + NADH + H+
-
 ?
1.2.1.88 benzaldehyde + NAD+ + H2O 2fold higher activity than with 1-pyrroline-5-carboxylate, isoform A, only slight activity with isoform B Hyalomma dromedarii benzoate + NADH + H+
-
 ?
1.2.1.88 D-glucose + NAD+ + H2O 80% of the activity with 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B Hyalomma dromedarii D-gluconate + NADH
-
 ?
1.2.1.88 formaldehyde + NAD+ + H2O 50% of activity with 1-pyrroline-5-carboxylate, isoform A, only slight activity with isoform B Hyalomma dromedarii formate + NADH
-
 ?
1.2.1.88 glyceralaldehyde-3-phosphate + NAD+ + H2O slight activity with isoforms A and B Hyalomma dromedarii glycerate-3-phosphate + NADH
-
 ?
1.2.1.88 glyoxylic acid + NAD+ + H2O 41% activity of that with 1-pyrroline-5-carboxylate with isoform A, no activity with isoform B Hyalomma dromedarii oxalic acid + NADH
-
 ?
1.2.1.88 indole-3-acetaldehyde + NAD+ + H2O 66% activity of that with 1-pyrroline-5-carboxylate with isoform A, only slight activity with isoform B Hyalomma dromedarii indole-3-acetate + NADH + H+
-
 ?
1.2.1.88 indole-3-phosphate + NAD+ + H2O slight activity with isoforms A and B Hyalomma dromedarii ?
-
 ?
1.2.1.88 additional information additional esterase activity with p-nitrophenyl acetate as substrate, 50% of activity with 1-pyrroline-5-carboxylate, isoform A, only slight activity with isoform B Hyalomma dromedarii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.88 monomer 1 * 59000, isoform A, SDS-PAGE Hyalomma dromedarii
1.2.1.88 monomer 1 * 52000, isoform B, SDS-PAGE Hyalomma dromedarii

Synonyms

EC Number Synonyms Comment Organism
1.2.1.88 P5CD
-
 Hyalomma dromedarii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.88 7.5
-
 both isoforms Hyalomma dromedarii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.88 2.5
-
 proline isoform B Hyalomma dromedarii
1.2.1.88 3
-
 gamma-aminobutyrate isoform B Hyalomma dromedarii
1.2.1.88 3
-
 proline isoform A Hyalomma dromedarii
1.2.1.88 3.4
-
 glutamate isoform B Hyalomma dromedarii
1.2.1.88 3.75
-
 hydroxyproline isoform B Hyalomma dromedarii
1.2.1.88 3.75
-
 gamma-aminobutyrate isoform A Hyalomma dromedarii
1.2.1.88 4.75
-
 hydroxyproline isoform A Hyalomma dromedarii
1.2.1.88 7
-
 glutamate isoform A Hyalomma dromedarii