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Literature summary extracted from

  • Mackenzie, R.E.
    Mitochondrial NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase (1997), Methods Enzymol., 281, 171-177.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.15 phosphate
-
Mus musculus
1.5.1.15 phosphate absolute requirement, phosphate can be replaced by its analog arsenate Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.15 cloned cDNA for the human enzyme expressed in Escherichia coli K-38 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.15 0.005
-
L-5,10-methylenetetrahydrofolate
-
Homo sapiens
1.5.1.15 0.013
-
NAD+
-
Spodoptera frugiperda
1.5.1.15 0.019
-
L-5,10-methylenetetrahydrofolate
-
Mus musculus
1.5.1.15 0.03
-
methenyltetrahydrofolate
-
Spodoptera frugiperda
1.5.1.15 0.096
-
methenyltetrahydrofolate
-
Homo sapiens
1.5.1.15 0.111
-
NAD+
-
Mus musculus
1.5.1.15 0.132
-
NAD+
-
Homo sapiens
1.5.1.15 0.155
-
5,10-methenyltetrahydrofolate
-
Mus musculus
1.5.1.15 0.17
-
phosphate
-
Mus musculus
1.5.1.15 0.19
-
phosphate
-
Homo sapiens
1.5.1.15 0.25
-
Mg2+
-
Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.15 cytoplasm
-
Drosophila melanogaster 5737
-
1.5.1.15 cytoplasm
-
Mus musculus 5737
-
1.5.1.15 cytoplasm
-
Homo sapiens 5737
-
1.5.1.15 cytoplasm
-
Saccharomyces cerevisiae 5737
-
1.5.1.15 cytoplasm
-
Spodoptera frugiperda 5737
-
1.5.1.15 mitochondrion
-
Drosophila melanogaster 5739
-
1.5.1.15 mitochondrion
-
Mus musculus 5739
-
1.5.1.15 mitochondrion
-
Homo sapiens 5739
-
1.5.1.15 mitochondrion
-
Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.15 Mg2+
-
Mus musculus
1.5.1.15 Mg2+
-
Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.15 34000
-
-
Mus musculus
1.5.1.15 34000
-
-
Homo sapiens
1.5.1.15 37000
-
-
Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Drosophila melanogaster
-
L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Mus musculus
-
L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Homo sapiens
-
L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Saccharomyces cerevisiae
-
L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Spodoptera frugiperda
-
L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Drosophila melanogaster
-
5,10-methenyl-tetrahydrofolate + NADH
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Mus musculus
-
5,10-methenyl-tetrahydrofolate + NADH
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Homo sapiens
-
5,10-methenyl-tetrahydrofolate + NADH
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Saccharomyces cerevisiae
-
5,10-methenyl-tetrahydrofolate + NADH
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Spodoptera frugiperda
-
5,10-methenyl-tetrahydrofolate + NADH
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.15 Drosophila melanogaster
-
-
-
1.5.1.15 Homo sapiens
-
-
-
1.5.1.15 Mus musculus
-
mouse, Ehrlich ascites tumor cells
-
1.5.1.15 Saccharomyces cerevisiae
-
-
-
1.5.1.15 Spodoptera frugiperda
-
fall armyworm, cell line Sf9
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.15
-
Spodoptera frugiperda
1.5.1.15 from ascites tumor cells Mus musculus
1.5.1.15 recombinant enzyme, expressed in Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.15 31.9
-
-
Mus musculus
1.5.1.15 40 44
-
Homo sapiens
1.5.1.15 43
-
-
Spodoptera frugiperda

Storage Stability

EC Number Storage Stability Organism
1.5.1.15 -80°C, enzyme is stable in 20-30% glycerol buffer and can be frozen for months without loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
Drosophila melanogaster L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
Mus musculus L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
Homo sapiens L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
Saccharomyces cerevisiae L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
Spodoptera frugiperda L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
Drosophila melanogaster 5,10-methenyl-tetrahydrofolate + NADH
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
Mus musculus 5,10-methenyl-tetrahydrofolate + NADH
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
Homo sapiens 5,10-methenyl-tetrahydrofolate + NADH
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
Saccharomyces cerevisiae 5,10-methenyl-tetrahydrofolate + NADH
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
Spodoptera frugiperda 5,10-methenyl-tetrahydrofolate + NADH
-
r