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Literature summary extracted from

  • Huennekens, F.M.; Vitols, K.S.; Whiteley, J.M.; Neef, V.G.
    Dihydrofolate reductase (1976), Methods Cancer Res., 13, 199-225.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 methotrexate
-
 Bos taurus
1.5.1.3 methotrexate
-
 Cricetulus sp.
1.5.1.3 methotrexate
-
 Enterococcus faecalis
1.5.1.3 methotrexate
-
 Escherichia coli
1.5.1.3 methotrexate
-
 Gallus gallus
1.5.1.3 methotrexate
-
 Lacticaseibacillus casei
1.5.1.3 methotrexate
-
 Mammalia
1.5.1.3 methotrexate
-
 Mus musculus
1.5.1.3 methotrexate
-
 Sus scrofa
1.5.1.3 methotrexate
-
 Tequatrovirus T4

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 additional information
-
 additional information
-
 Mammalia
1.5.1.3 additional information
-
 additional information
-
 Enterococcus faecalis
1.5.1.3 additional information
-
 additional information
-
 Tequatrovirus T4
1.5.1.3 additional information
-
 additional information overview Gallus gallus
1.5.1.3 additional information
-
 additional information overview Mus musculus
1.5.1.3 additional information
-
 additional information overview Escherichia coli
1.5.1.3 additional information
-
 additional information overview Sus scrofa
1.5.1.3 additional information
-
 additional information overview Bos taurus
1.5.1.3 additional information
-
 additional information overview Lacticaseibacillus casei
1.5.1.3 additional information
-
 additional information overview Enterococcus faecium
1.5.1.3 additional information
-
 additional information overview Cricetulus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 additional information
-
 overview Gallus gallus
1.5.1.3 additional information
-
 overview Mammalia
1.5.1.3 additional information
-
 overview Mus musculus
1.5.1.3 additional information
-
 overview Sus scrofa
1.5.1.3 additional information
-
 overview Bos taurus
1.5.1.3 additional information
-
 overview Cricetulus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH Mammalia
-
 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH Gallus gallus maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH Sus scrofa maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH Bos taurus maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH Cricetulus sp. maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Bos taurus
-
 calf
-
1.5.1.3 Cricetulus sp.
-
 baby hamster kidney cells
-
1.5.1.3 Enterococcus faecalis
-
-
-
1.5.1.3 Enterococcus faecium
-
-
-
1.5.1.3 Escherichia coli
-
 amethopterin-resistant
-
1.5.1.3 Gallus gallus
-
-
-
1.5.1.3 Lacticaseibacillus casei
-
 amethopterin-resistant
-
1.5.1.3 Mammalia
-
-
-
1.5.1.3 Mus musculus
-
 L1210 lymphoma cells, amethopterin-resistant
-
1.5.1.3 Sus scrofa
-
-
-
1.5.1.3 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 overview Mus musculus
1.5.1.3 overview Sus scrofa
1.5.1.3 overview Bos taurus
1.5.1.3 overview Enterococcus faecalis
1.5.1.3 overview Enterococcus faecium
1.5.1.3 overview Tequatrovirus T4
1.5.1.3 overview Cricetulus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.3 kidney
-
 Cricetulus sp.
-
1.5.1.3 liver
-
 Gallus gallus
-
1.5.1.3 liver
-
 Mus musculus
-
1.5.1.3 liver
-
 Sus scrofa
-
1.5.1.3 liver
-
 Bos taurus
-
1.5.1.3 thymus
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 additional information
-
-
 Escherichia coli
1.5.1.3 additional information
-
-
 Lacticaseibacillus casei
1.5.1.3 additional information
-
-
 Tequatrovirus T4
1.5.1.3 additional information
-
 enzyme assay Gallus gallus
1.5.1.3 additional information
-
 enzyme assay Sus scrofa
1.5.1.3 additional information
-
 enzyme assay Bos taurus
1.5.1.3 additional information
-
 enzyme assay Cricetulus sp.
1.5.1.3 additional information
-
 overview Gallus gallus
1.5.1.3 additional information
-
 overview Mus musculus
1.5.1.3 additional information
-
 overview Sus scrofa
1.5.1.3 additional information
-
 overview Bos taurus
1.5.1.3 additional information
-
 overview Cricetulus sp.
1.5.1.3 11.6
-
 amethopterin-resistant cells L1210/S Mus musculus
1.5.1.3 23
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Gallus gallus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Mammalia 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Mus musculus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Sus scrofa 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Bos taurus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Bos taurus 5,6,7,8-tetrahydrofolate + NADP+
-
 r
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Cricetulus sp. 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH equilibrium strongly favors tetrahydrofolate production Mammalia 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH equilibrium strongly favors tetrahydrofolate production Mammalia 5,6,7,8-tetrahydrofolate + NADP+
-
 r
1.5.1.3 7,8-dihydrofolate + NADPH equilibrium strongly favors tetrahydrofolate production Escherichia coli 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH equilibrium strongly favors tetrahydrofolate production Escherichia coli 5,6,7,8-tetrahydrofolate + NADP+
-
 r
1.5.1.3 7,8-dihydrofolate + NADPH equilibrium strongly favors tetrahydrofolate production Enterococcus faecalis 5,6,7,8-tetrahydrofolate + NADP+
-
 r
1.5.1.3 7,8-dihydrofolate + NADPH equilibrium strongly favors tetrahydrofolate production Lacticaseibacillus casei 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH equilibrium strongly favors tetrahydrofolate production Lacticaseibacillus casei 5,6,7,8-tetrahydrofolate + NADP+
-
 r
1.5.1.3 7,8-dihydrofolate + NADPH equilibrium strongly favors tetrahydrofolate production Enterococcus faecium 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH equilibrium strongly favors tetrahydrofolate production Enterococcus faecium 5,6,7,8-tetrahydrofolate + NADP+
-
 r
1.5.1.3 7,8-dihydrofolate + NADPH maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells Gallus gallus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells Sus scrofa 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells Bos taurus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells Cricetulus sp. 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 folate + NADPH
-
 Gallus gallus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 folate + NADPH
-
 Mus musculus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 folate + NADPH
-
 Sus scrofa 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 folate + NADPH
-
 Bos taurus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 folate + NADPH
-
 Cricetulus sp. 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 folate + NADPH at a slow rate, reaction not catalyzed by all dihydrofolate reductases Mammalia 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 additional information
-
 Escherichia coli ?
-
 ?
1.5.1.3 additional information equilibrium strongly favors tetrahydrofolate production Gallus gallus ?
-
 ?
1.5.1.3 additional information equilibrium strongly favors tetrahydrofolate production Sus scrofa ?
-
 ?
1.5.1.3 additional information equilibrium strongly favors tetrahydrofolate production Bos taurus ?
-
 ?
1.5.1.3 additional information equilibrium strongly favors tetrahydrofolate production Cricetulus sp. ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.3 additional information
-
 overview Gallus gallus
1.5.1.3 additional information
-
 overview Mus musculus
1.5.1.3 additional information
-
 overview Sus scrofa
1.5.1.3 additional information
-
 overview Bos taurus
1.5.1.3 additional information
-
 overview Cricetulus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 additional information
-
-
 Escherichia coli
1.5.1.3 additional information
-
 overview Gallus gallus
1.5.1.3 additional information
-
 overview Mus musculus
1.5.1.3 additional information
-
 overview Sus scrofa
1.5.1.3 additional information
-
 overview Bos taurus
1.5.1.3 additional information
-
 overview Cricetulus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.3 additional information
-
-
 Enterococcus faecium
1.5.1.3 additional information
-
-
 Cricetulus sp.
1.5.1.3 additional information
-
 overview Mammalia

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Gallus gallus
1.5.1.3 NADPH
-
 Mammalia
1.5.1.3 NADPH
-
 Mus musculus
1.5.1.3 NADPH
-
 Escherichia coli
1.5.1.3 NADPH
-
 Sus scrofa
1.5.1.3 NADPH
-
 Bos taurus
1.5.1.3 NADPH
-
 Enterococcus faecalis
1.5.1.3 NADPH
-
 Lacticaseibacillus casei
1.5.1.3 NADPH
-
 Enterococcus faecium
1.5.1.3 NADPH
-
 Tequatrovirus T4
1.5.1.3 NADPH
-
 Cricetulus sp.