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Literature summary extracted from

  • Iwai, K.; Kohashi, M.; Oe, H.
    Purification and properties of dihydrofolate reductase from Crithidia fasciculata (1981), Agric. Biol. Chem., 45, 113-120.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.5.1.3 2-mercaptoethanol, 4 mM, stabilizes purified preparation, inclusion in purification steps causes loss of activity Crithidia fasciculata
1.5.1.3 glycerol, 30%, stabilization of purified preparation Crithidia fasciculata

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 aminopterin
-
 Crithidia fasciculata
1.5.1.3 chaotrophes e.g. guanidine-HCl; formamide; urea Crithidia fasciculata
1.5.1.3 K+
-
 Crithidia fasciculata
1.5.1.3 methotrexate
-
 Crithidia fasciculata
1.5.1.3 N-ethylmaleimide
-
 Crithidia fasciculata
1.5.1.3 p-chloromercuribenzoate
-
 Crithidia fasciculata
1.5.1.3 pyrimethamine
-
 Crithidia fasciculata
1.5.1.3 trimethoprim
-
 Crithidia fasciculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.0011
-
 7,8-dihydrofolate
-
 Crithidia fasciculata
1.5.1.3 0.0027
-
 NADPH
-
 Crithidia fasciculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 58000
-
 2 * 58000, isoform IIa, SDS-PAGE Crithidia fasciculata
1.5.1.3 110000
-
 isoform IIa, gel filtration Crithidia fasciculata

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Crithidia fasciculata
-
 three different forms I, IIa and IIb
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 isoform IIa Crithidia fasciculata

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.5.1.3 denaturation with 4 M urea, no renaturation by removing urea by dialysis Crithidia fasciculata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 6.8
-
-
 Crithidia fasciculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH reverse reaction at only one tenth of forward reaction Crithidia fasciculata 5,6,7,8-tetrahydrofolate + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.1.3 dimer 2 * 58000, isoform IIa, SDS-PAGE Crithidia fasciculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 7
-
-
 Crithidia fasciculata

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADH not Crithidia fasciculata
1.5.1.3 NADPH
-
 Crithidia fasciculata