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Literature summary extracted from

  • Rancourt, S.L.; Walker, V.K.
    The purification of dihydrofolate reductase from Drosophila melanogaster (1990), Biochim. Biophys. Acta, 1039, 261-268.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.3 stabilized by 0.1 mg/ml bovine serum albumin and 1 mM dithiothreitol at 4°C Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.3 soluble
-
 Drosophila melanogaster
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 17000
-
 gel filtration Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
 Drosophila melanogaster

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 5.3
-
 purified enzyme Drosophila melanogaster

Storage Stability

EC Number Storage Stability Organism
1.5.1.3 on ice, loss of 20-60% activity after 2 h Drosophila melanogaster

Subunits

EC Number Subunits Comment Organism
1.5.1.3 monomer 1 * 17000-22000 Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 4.7
-
 2 optima: pH 4.7 and pH 8.5 Drosophila melanogaster
1.5.1.3 8.5
-
 2 optima: pH 4.7 and pH 8.5 Drosophila melanogaster

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.3 4.5 9
-
 Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Drosophila melanogaster