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Literature summary extracted from

  • Toth, I.; Lazar, G.; Goodman, H.M.
    Purification and immunochemical charaterization of a dihydrofolate reductase-thymidylate synthase enzyme complex from wild-carrot cells (1987), EMBO J., 6, 1853-1858.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 5,5'-dithiobis(2-nitrobenzoate)
-
Daucus carota
1.5.1.3 aminopterin
-
Daucus carota
1.5.1.3 methotrexate
-
Daucus carota
1.5.1.3 N-ethylmaleimide
-
Daucus carota
1.5.1.3 N10-formylaminopterin
-
Daucus carota
1.5.1.3 p-hydroxymercuribenzoate
-
Daucus carota

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 45000
-
enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function, dihydrofolate reductase subunit, SDS-PAGE Daucus carota

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Daucus carota
-
wild carrot, enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function Daucus carota

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 10.8
-
purified enzyme Daucus carota

Subunits

EC Number Subunits Comment Organism
1.5.1.3 More enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function, 286000 MW complex, 45000 MW dihydrofolate reductase subunit and 4 other polypeptide chains: 95000, 70000, 50000, 26000 Daucus carota

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.3 42
-
assay at Daucus carota

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.3 additional information
-
assay at pH 7.2, no activity at pH 5.9 Daucus carota