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Literature summary extracted from
- Dihydrofolate reductase from bovine liver. Enzymatic and structural properties (1975), Eur. J. Biochem., 60, 9-15.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.5.1.3 | 7,8-dihydrofolate protects against inactivation | Bos taurus |
| 1.5.1.3 | unstable to dialysis and lyophilization and concentration by membrane filtration under nitrogen | Bos taurus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.3 | 23000 | - |
SDS-PAGE in presence of 2-mercaptoethanol | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.3 | additional information | Bos taurus | key enzyme in the biosynthesis of purines, pyrimidines and several amino acids | ? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.1.3 | Bos taurus | - |
2 forms | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.1.3 | 2 forms | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.5.1.3 | liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.3 | 29.69 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.3 | 7,8-dihydrofolate + NADPH | - |
Bos taurus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 1.5.1.3 | additional information | key enzyme in the biosynthesis of purines, pyrimidines and several amino acids | Bos taurus | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.3 | 4 | - |
above, for extended length of time, unstable | Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.3 | NADPH | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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