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Literature summary extracted from

  • Rios-Orlandi, E.M.; Zarkadas, C.G.; MacKenzie, R.E.
    Formyltetrahydrofolate dehydrogenase-hydrolase from pig liver: simultaneous assay of the activities (1986), Biochim. Biophys. Acta, 871, 24-35.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.6 tetrahydrofolate very strong product inhibition Sus scrofa
1.5.1.6 tetrahydropteroylpolyglutamate inhibition increases with increasing length of polyglutamyl chain Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.6 0.0004
-
 NADP+ spectrophotometric assay Sus scrofa
1.5.1.6 0.0006
-
 NADP+ fluorimetric assay Sus scrofa
1.5.1.6 0.0044
-
 10-formyltetrahydrofolate racemic, fluorimetric assay Sus scrofa
1.5.1.6 0.0075
-
 10-formyltetrahydrofolate racemic, spectrophotometric assay Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.6 soluble
-
 Sus scrofa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.6 92500
-
 4 * 92500, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O Sus scrofa major disposal route for excess one-carbon units produced in folate-mediated metabolism tetrahydrofolate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.6 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.6
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.6 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.6 0.64
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.5.1.6 -20°C, dialyzed against 0.01 M potassium phosphate buffer, pH 7.3, 40% glycerol, 0.1 M KCl, several weeks, stable Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O specific for NADP+ Sus scrofa tetrahydrofolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O major disposal route for excess one-carbon units produced in folate-mediated metabolism Sus scrofa tetrahydrofolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 10-formyltetrahydropteroylpolyglutamate + NADP+ + H2O
-
 Sus scrofa tetrahydropteroylpolyglutamate + CO2 + NADPH + H+
-
 ?
1.5.1.6 additional information bifunctional enzyme: 10-formyltetrahydrofolate dehydrogenase separated from hydrolase activity, activities are functions of different active sites and take place simultaneously in presence of NADP+ Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.6 homotetramer 4 * 92500, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.6 25
-
 assay at Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.6 1
-
 10-formyltetrahydrofolate
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.6 10-formyltetrahydrofolate 10-formyltetrahydrofolate Sus scrofa
1.5.1.6 NADP+ NADP+-dependent Sus scrofa
1.5.1.6 tetrahydrofolate
-
 Sus scrofa