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Literature summary extracted from

  • Hutzler, J.; Dancis, J.
    Lysine-ketoglutarate reductase in human tissues (1975), Biochim. Biophys. Acta, 377, 42-51.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.8 EDTA 1 mM, slight activation, effect not consistant Homo sapiens
1.5.1.8 Triton X-100 1%, slight activation, effect not consistant Homo sapiens

Application

EC Number Application Comment Organism
1.5.1.8 medicine hyperlysinemia: genetic disease, deficiency of lysine-ketoglutarate reductase results in extreme elevations of serum lysine Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.8 CaCl2 3 mM, 28% loss of activity Homo sapiens
1.5.1.8 cadaverine 3 mM, 26% loss of activity Homo sapiens
1.5.1.8 Carbamoylphosphate 3 mM, 25% loss of activity Homo sapiens
1.5.1.8 CoCl2 1 mM, 82% loss of activity Homo sapiens
1.5.1.8 CuSO4 1 mM, 87% loss of activity Homo sapiens
1.5.1.8 DL-pipecolic acid 6 mM, 47% loss of activity Homo sapiens
1.5.1.8 HgCl2 0.01 mM, 89% loss of activity Homo sapiens
1.5.1.8 hydroxylamine 3 mM, 33% loss of activity Homo sapiens
1.5.1.8 L-Glutamic acid 3 mM, 40% loss of activity Homo sapiens
1.5.1.8 L-Homocitrulline 3 mM, 49% loss of activity Homo sapiens
1.5.1.8 L-Lysylglycine 3 mM, 25% loss of activity Homo sapiens
1.5.1.8 L-ornithine 3 mM, 39% loss of activity Homo sapiens
1.5.1.8 leucine
-
Homo sapiens
1.5.1.8 MgSO4 1 mM, 79% loss of activity Homo sapiens
1.5.1.8 MnCl2 1 mM, 52% loss of activity Homo sapiens
1.5.1.8 additional information not inhibited by 40 mM ammonium chloride, inhibition by NH4+ is probably due to contamination with glutamic dehydrogenase Homo sapiens
1.5.1.8 saccharopine 1 mM, 30% inhibition, product inhibition Homo sapiens
1.5.1.8 ZnCl2 3 mM, complete inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.8 0.08
-
NADPH
-
Homo sapiens
1.5.1.8 1
-
2-oxoglutarate
-
Homo sapiens
1.5.1.8 1.5
-
L-lysine
-
Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH Homo sapiens saccharopine pathway is the major route of lysine breakdown N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
?
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Homo sapiens involved in lysine catabolism L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.8 Homo sapiens
-
-
-
1.5.1.9 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.8 44.6fold partial purification from liver Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.8 heart after liver next highest enzyme concentration Homo sapiens
-
1.5.1.8 kidney low enzyme concentration Homo sapiens
-
1.5.1.8 liver highest enzyme concentration Homo sapiens
-
1.5.1.8 additional information trace enzyme activity in muscle, brain, and spleen, no activity in pancreas, adrenal, lung, gut, leucocytes, erythrocytes Homo sapiens
-
1.5.1.8 skin low enzyme concentration Homo sapiens
-
1.5.1.9 kidney
-
Homo sapiens
-
1.5.1.9 liver
-
Homo sapiens
-
1.5.1.9 additional information no activity in: human heart, muscle, skin, brain, spleen, pancreas, adrenal, lung, gut, leukocytes and erythrocytes Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.8 additional information
-
-
Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.5.1.8 -20°C, 1 month, 50% loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.8 DL-delta-hydroxylysine + 2-oxoglutarate + NADPH 18% of velocity with equimolar concentration of L-lysine Homo sapiens ?
-
?
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH reverse reaction not detected Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ir
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ir
1.5.1.8 L-lysine + 2-oxoglutarate + NADPH saccharopine pathway is the major route of lysine breakdown Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
?
1.5.1.8 additional information less than 2% of the rate of L-lysine: D-lysine, cadaverine, delta-aminovaleric acid, alpha-acetyl-L-lysine, alpha-amino-epsilon-hydroxycaproic acid, L-lysylglycine, L-2,4-diaminobutyric acid, DL-pipecolic acid, L-ornithine, epsilon-aminocaproic acid, 4% of the rate of L-lysine: DL-alpha,epsilon-diaminopimelic acid, 7% of the rate of 2-oxoglutarate: oxaloacetic acid, less than 2% of the rate of 2-oxoglutarate: 2-oxoadipate, pyruvate, 2-oxoisocaproate, 2-oxoisovalerate, levulinate, 2-hydroxyglutarate Homo sapiens ?
-
?
1.5.1.8 S-2-aminoethyl-L-cysteine + 2-oxoglutarate + NADPH 47% of velocity with equimolar concentration of L-lysine Homo sapiens ?
-
?
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
Homo sapiens L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
r
1.5.1.9 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O involved in lysine catabolism Homo sapiens L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.8 23
-
assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.8 40
-
10 min, unstable above Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.8 7.8
-
-
Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.8 7.2 8.8 about 50% of activity maximum at pH 7.2 and 8.8 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.1.8 4.4
-
10 min, 50% loss of activity Homo sapiens
1.5.1.8 4.6 4.9 10 min, stable Homo sapiens
1.5.1.9 4.4
-
10 min, 60% activity Homo sapiens
1.5.1.9 4.6 4.9 10 min Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.8 NADPH
-
Homo sapiens
1.5.1.9 NAD+
-
Homo sapiens