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Literature summary extracted from

  • Inouye, Y.; Nishimura, M.; Matsuda, Y.; Hoshika, H.; Iwasaki, H.; Hujimura, K.; Asano, K.; Nakamura, S.
    Purification and characterization of sarcosine oxidase of Streptomyces origin (1987), Chem. Pharm. Bull., 35, 4194-4202.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.1 EDTA not Streptomyces sp.
1.5.3.1 Hydroxylamine hydrochloride
-
Streptomyces sp.
1.5.3.1 N-bromosuccinimide
-
Streptomyces sp.
1.5.3.1 Ni2+
-
Streptomyces sp.
1.5.3.1 p-chloromercuribenzoate not inhibitory Streptomyces sp.
1.5.3.1 SDS
-
Streptomyces sp.
1.5.3.1 Zn2+
-
Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.1 0.58
-
N-Methyl-L-leucine
-
Streptomyces sp.
1.5.3.1 0.91
-
sarcosine
-
Streptomyces sp.
1.5.3.1 1.6
-
N-Methyl-DL-alanine
-
Streptomyces sp.
1.5.3.1 6.7
-
N-Methyl-DL-valine
-
Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.1 44000
-
gel filtration, SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.1 sarcosine + H2O + O2 Streptomyces sp. involved in creatinine catabolism ?
-
?
1.5.3.1 sarcosine + H2O + O2 Streptomyces sp. KB210-8SY involved in creatinine catabolism ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.1 Streptomyces sp.
-
KB210-8SY
-
1.5.3.1 Streptomyces sp. KB210-8SY
-
KB210-8SY
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.1
-
Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.1 25
-
-
Streptomyces sp.

Storage Stability

EC Number Storage Stability Organism
1.5.3.1 frozen Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.1 N-methyl-DL-alanine + O2 + H2O
-
Streptomyces sp. formaldehyde + DL-alanine + H2O2
-
?
1.5.3.1 N-methyl-DL-alanine + O2 + H2O
-
Streptomyces sp. KB210-8SY formaldehyde + DL-alanine + H2O2
-
?
1.5.3.1 N-methyl-DL-valine + O2 + H2O
-
Streptomyces sp. formaldehyde + DL-valine + H2O2
-
?
1.5.3.1 N-methyl-DL-valine + O2 + H2O
-
Streptomyces sp. KB210-8SY formaldehyde + DL-valine + H2O2
-
?
1.5.3.1 N-methyl-L-leucine + O2 + H2O
-
Streptomyces sp. formaldehyde + L-leucine + H2O2
-
?
1.5.3.1 N-methyl-L-leucine + O2 + H2O
-
Streptomyces sp. KB210-8SY formaldehyde + L-leucine + H2O2
-
?
1.5.3.1 sarcosine + H2O + O2 involved in creatinine catabolism Streptomyces sp. ?
-
?
1.5.3.1 sarcosine + H2O + O2 involved in creatinine catabolism Streptomyces sp. KB210-8SY ?
-
?
1.5.3.1 sarcosine + O2 + H2O
-
Streptomyces sp. glycine + formaldehyde + H2O2
-
?
1.5.3.1 sarcosine + O2 + H2O
-
Streptomyces sp. KB210-8SY glycine + formaldehyde + H2O2
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.1 8
-
-
Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.3.1 6 10 pH 10: about 80% of maximum activity Streptomyces sp.
1.5.3.1 6 10 pH 6: about 65% of maximum activity Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.3.1 7 9
-
Streptomyces sp.