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Literature summary extracted from

  • Suzuki, M.
    Purification and some properties of sarcosine oxidase from Corynebacterium sp. U-96 (1981), J. Biochem., 89, 599-607.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.1 acetaldehyde
-
 Corynebacterium sp.
1.5.3.1 acetate
-
 Corynebacterium sp.
1.5.3.1 AgNO3 2 mM, 0% relative activity Corynebacterium sp.
1.5.3.1 Cd2+ 2 mM, 7% relative activity Corynebacterium sp.
1.5.3.1 Cu2+ 2 mM, 0% relative activity Corynebacterium sp.
1.5.3.1 Fe3+ not inhibitory Corynebacterium sp.
1.5.3.1 formaldehyde
-
 Corynebacterium sp.
1.5.3.1 Hg2+ 2 mM, 0% relative activity Corynebacterium sp.
1.5.3.1 iodoacetamide 2 mM, 4% relative activity Corynebacterium sp.
1.5.3.1 iodoacetate
-
 Corynebacterium sp.
1.5.3.1 N-ethylmaleimide 2 mM, 20% relative activity Corynebacterium sp.
1.5.3.1 p-chloromercuribenzoate 0.01 mM, 0% relative activity Corynebacterium sp.
1.5.3.1 propionate
-
 Corynebacterium sp.
1.5.3.1 sodium lauryl sulfate 2 mM, 0% relative activity Corynebacterium sp.
1.5.3.1 Zn2+ 2 mM, 7% relative activity Corynebacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.1 0.13
-
 O2
-
 Corynebacterium sp.
1.5.3.1 0.31
-
 2,6-dichlorophenolindophenol
-
 Corynebacterium sp.
1.5.3.1 1.67
-
 phenazine methosulfate
-
 Corynebacterium sp.
1.5.3.1 2.7
-
 potassium ferricyanide
-
 Corynebacterium sp.
1.5.3.1 3.4
-
 sarcosine
-
 Corynebacterium sp.
1.5.3.1 8.7
-
 N-Methyl-DL-alanine
-
 Corynebacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.1 10000
-
 1 * 110000 (A) + 1 * 44000 (B) + 1 * 21000 (C) + 1 * 10000 (D), SDS-PAGE Corynebacterium sp.
1.5.3.1 21000
-
 1 * 110000 (A) + 1 * 44000 (B) + 1 * 21000 (C) + 1 * 10000 (D), SDS-PAGE Corynebacterium sp.
1.5.3.1 44000
-
 1 * 110000 (A) + 1 * 44000 (B) + 1 * 21000 (C) + 1 * 10000 (D), SDS-PAGE Corynebacterium sp.
1.5.3.1 110000
-
 1 * 110000 (A) + 1 * 44000 (B) + 1 * 21000 (C) + 1 * 10000 (D), SDS-PAGE Corynebacterium sp.
1.5.3.1 174000
-
 meniscus depletion method Corynebacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.1 Corynebacterium sp.
-
 U-96
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.1 U-96 Corynebacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.1 8.93
-
-
 Corynebacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.1 additional information very specific for oxygen as acceptor, oxygen can be replaced by 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, much smaller Vmax/Km values than for O2 Corynebacterium sp. ?
-
 ?
1.5.3.1 N-ethylglycine + O2 + H2O
-
 Corynebacterium sp. acetaldehyde + glycine + H2O2
-
 ?
1.5.3.1 N-methyl-DL-alanine + O2 + H2O
-
 Corynebacterium sp. formaldehyde + DL-alanine + H2O2
-
 ?
1.5.3.1 N-methyl-L-alanine + O2 + H2O
-
 Corynebacterium sp. formaldehyde + L-alanine + H2O2
-
 r
1.5.3.1 sarcosine + H2O + 2,6-dichlorophenolindophenol
-
 Corynebacterium sp. glycine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 r
1.5.3.1 sarcosine + H2O + phenazine methosulfate
-
 Corynebacterium sp. glycine + formaldehyde + reduced phenazine methosulfate
-
 r
1.5.3.1 sarcosine + H2O + potassium ferricyanide
-
 Corynebacterium sp. glycine + formaldehyde + potassium ferrocyanide
-
 r
1.5.3.1 sarcosine + O2 + H2O
-
 Corynebacterium sp. glycine + formaldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.1 tetramer 1 * 110000 (A) + 1 * 44000 (B) + 1 * 21000 (C) + 1 * 10000 (D), SDS-PAGE Corynebacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.3.1 45
-
 10 min, complete loss of activity Corynebacterium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.1 2.2
-
 N-ethylglycine
-
 Corynebacterium sp.
1.5.3.1 3.4
-
 N-Methyl-L-alanine
-
 Corynebacterium sp.
1.5.3.1 5.8
-
 sarcosine
-
 Corynebacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.3.1 6 10 pH 6: about 35% of maximum activity Corynebacterium sp.
1.5.3.1 6 10 pH 10: about 70% of maximum activity Corynebacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.1 flavin 1 mol of covalently bound and 1 mol of noncovalently bound flavin per mol of enzyme Corynebacterium sp.