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Literature summary extracted from
- Gene structures and properties of enzymes of the plasmid-encoded nicotine catabolism of Arthrobacter nicotinovorans (1998), J. Mol. Biol., 284, 1323-1339.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.5.3.5 | expressed in Escherichia coli JM105 | Paenarthrobacter nicotinovorans |
| 1.5.3.6 | expressed in Escherichia coli HB101 as a 6-hydroxy-D-nicotine oxidase/glutathione S-transferase fusion protein | Paenarthrobacter nicotinovorans |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.5.3.5 | - |
Paenarthrobacter nicotinovorans |
| 1.5.3.6 | fusion protein with glutathione S-transferase | Paenarthrobacter nicotinovorans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.3.6 | 6-hydroxy-L-nicotine | competitive inhibitor | Paenarthrobacter nicotinovorans |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.3.6 | 0.05 | - |
6-hydroxy-D-nicotine | - |
Paenarthrobacter nicotinovorans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.99.4 | Molybdenum | enzyme synthesis responds to the presence of this metal ion | Paenarthrobacter nicotinovorans | |
| 1.5.99.4 | Tungsten | enzyme synthesis responds to the presence of this metal ion | Paenarthrobacter nicotinovorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.3.5 | 46265 | - |
2 * 46265, amino acid analysis | Paenarthrobacter nicotinovorans |
| 1.5.3.6 | 48980 | - |
with FAD, amino acid analysis | Paenarthrobacter nicotinovorans |
| 1.5.99.4 | 290000 | - |
native gel electrophoresis | Paenarthrobacter nicotinovorans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.3.5 | (S)-6-hydroxynicotine + H2O + O2 | Paenarthrobacter nicotinovorans | - |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | transitional product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously | ? | |
| 1.5.3.6 | (R)-6-hydroxynicotine + H2O + O2 | Paenarthrobacter nicotinovorans | - |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | transcient product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously | ? | |
| 1.5.99.4 | DL-nicotine + ? | Paenarthrobacter nicotinovorans | first enzyme in nicotine pathway | 6-hydroxynicotine + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.3.5 | Paenarthrobacter nicotinovorans | - |
formerly Arthrobacter oxidans | - |
| 1.5.3.6 | Paenarthrobacter nicotinovorans | - |
strain DSM 420 | - |
| 1.5.99.4 | Paenarthrobacter nicotinovorans | - |
DSM 420, formerly Arthrobacter oxidans | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.3.5 | - |
Paenarthrobacter nicotinovorans |
| 1.5.3.6 | - |
Paenarthrobacter nicotinovorans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.3.6 | 32 | - |
- |
Paenarthrobacter nicotinovorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.3.5 | (S)-6-hydroxynicotine + H2O + O2 | - |
Paenarthrobacter nicotinovorans | 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | transitional product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously | ? | |
| 1.5.3.6 | (R)-6-hydroxynicotine + H2O + O2 | - |
Paenarthrobacter nicotinovorans | 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | transcient product is 6-hydroxy-N-methylmyosmine that hydrolyses spontaneously | ? | |
| 1.5.99.4 | DL-nicotine + ? | first enzyme in nicotine pathway | Paenarthrobacter nicotinovorans | 6-hydroxynicotine + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.3.5 | homodimer | 2 * 46265, amino acid analysis | Paenarthrobacter nicotinovorans |
| 1.5.3.6 | monomer | - |
Paenarthrobacter nicotinovorans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.5.3.5 | 9.2 | - |
assay at | Paenarthrobacter nicotinovorans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.3.5 | FAD | 1 FAD per subunit, non-covalently bound | Paenarthrobacter nicotinovorans | |
| 1.5.3.6 | FAD | covalently bound to a histidine | Paenarthrobacter nicotinovorans | |
| 1.5.99.4 | additional information | flavoprotein | Paenarthrobacter nicotinovorans |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.3.6 | 0.02 | - |
6-hydroxy-L-nicotine | - |
Paenarthrobacter nicotinovorans | |
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