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Literature summary extracted from
- Purification and properties of L-6-hydroxynicotine oxidase (1968), Eur. J. Biochem., 4, 95-102.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.5.3.5 | forms hexagonal crystals in ammonium sulfate solution | Paenarthrobacter nicotinovorans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.3.5 | HgCl2 | 100% inhibition at 0.005 mM | Paenarthrobacter nicotinovorans |  |
| 1.5.3.5 | Na2MoO4 | 12% inhibition at 0.05 mM | Paenarthrobacter nicotinovorans | |
| 1.5.3.5 | o-phenanthroline | 69% inhibition at 9 mM | Paenarthrobacter nicotinovorans | |
| 1.5.3.5 | p-chloromercuriphenylsulfonate | 60% inhibition at 0.025 mM, inhibition can be reversed by an excess of thiol compounds | Paenarthrobacter nicotinovorans | |
| 1.5.3.5 | Urea | at 4 M, 38% inhibition and at 7.2 M, 93% inhibition | Paenarthrobacter nicotinovorans | |
| 1.5.3.5 | ZnSO4 | 57% inhibition at 0.05 mM | Paenarthrobacter nicotinovorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.3.5 | 47000 | - |
2 * 47000, sedimentation equilibrium | Paenarthrobacter nicotinovorans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.3.5 | (S)-6-hydroxynicotine + H2O + O2 | Paenarthrobacter nicotinovorans | - |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? | |
| 1.5.3.5 | additional information | Paenarthrobacter nicotinovorans | enzyme production is induced by growing cells in D,L-nicotine as only source of carbon and nitrogen | ? | - |
? | |
| 1.5.3.6 | (R)-6-hydroxynicotine + H2O + O2 | Paenarthrobacter nicotinovorans | - |
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.3.5 | Paenarthrobacter nicotinovorans | - |
formerly Arthrobacter oxidans | - |
| 1.5.3.6 | Paenarthrobacter nicotinovorans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.3.5 | - |
Paenarthrobacter nicotinovorans |
| 1.5.3.6 | - |
Paenarthrobacter nicotinovorans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.3.5 | 4.73 | - |
- |
Paenarthrobacter nicotinovorans |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.5.3.5 | 0°C, saturated ammonium sulfate solution, 2 weeks, little inactivation | Paenarthrobacter nicotinovorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.3.5 | (S)-6-hydroxynicotine + H2O + O2 | - |
Paenarthrobacter nicotinovorans | 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? | |
| 1.5.3.5 | additional information | enzyme production is induced by growing cells in D,L-nicotine as only source of carbon and nitrogen | Paenarthrobacter nicotinovorans | ? | - |
? | |
| 1.5.3.6 | (R)-6-hydroxynicotine + H2O + O2 | - |
Paenarthrobacter nicotinovorans | 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.3.5 | homodimer | 2 * 47000, sedimentation equilibrium | Paenarthrobacter nicotinovorans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.3.5 | 40 | - |
75% activity after 15 min | Paenarthrobacter nicotinovorans |
| 1.5.3.5 | 50 | - |
9% activity after 5 min | Paenarthrobacter nicotinovorans |
| 1.5.3.5 | 60 | - |
2% activity after 5 min | Paenarthrobacter nicotinovorans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.3.5 | 29.3 | - |
6-hydroxy-L-nicotine | - |
Paenarthrobacter nicotinovorans | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.5.3.5 | 7.5 | - |
assay at | Paenarthrobacter nicotinovorans |
| 1.5.3.6 | 9.2 | - |
assay at | Paenarthrobacter nicotinovorans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.5.3.5 | 6 | 9 | very unstable under pH 6 | Paenarthrobacter nicotinovorans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.3.5 | FAD | 1 FAD per subunit, non-covalently bound | Paenarthrobacter nicotinovorans | |
| 1.5.3.6 | FAD | - |
Paenarthrobacter nicotinovorans | |
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