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Literature summary extracted from

  • Bruhmuller, M.; Möhler, H.; Decker, K.
    Covalently bound flavin in D-6-hydroxynicotine oxidase from Arthrobacter oxidans. Purification and properties of D-6-hydroxynicotine oxidase (1972), Eur. J. Biochem., 29, 143-151.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.6 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one
-
Paenarthrobacter nicotinovorans
1.5.3.6 3-(4-aminobutyl)-pyridine
-
Paenarthrobacter nicotinovorans
1.5.3.6 6-hydroxy-L-nicotine
-
Paenarthrobacter nicotinovorans
1.5.3.6 D,L-2-Hydroxynicotine
-
Paenarthrobacter nicotinovorans
1.5.3.6 Phenanthroline inactivation above 1 mM Paenarthrobacter nicotinovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.6 0.05
-
6-hydroxy-D-nicotine
-
Paenarthrobacter nicotinovorans
1.5.3.6 0.2
-
6-amino-D-nicotine
-
Paenarthrobacter nicotinovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.6 53000
-
sedimentation equilibrium and SDS-PAGE Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.6 (R)-6-hydroxynicotine + H2O + O2 Paenarthrobacter nicotinovorans
-
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
?
1.5.3.6 additional information Paenarthrobacter nicotinovorans
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.6 Paenarthrobacter nicotinovorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.6
-
Paenarthrobacter nicotinovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.6 22.5
-
-
Paenarthrobacter nicotinovorans

Storage Stability

EC Number Storage Stability Organism
1.5.3.6 4°C, fairly stable at neutral or alkaline pH in presence of 10 mM mercaptoethanol Paenarthrobacter nicotinovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.6 (R)-6-aminonicotine + H2O + O2
-
Paenarthrobacter nicotinovorans 1-(-aminopyridin-3-yl)-4-methylamino-butan-1-one + H2O2
-
?
1.5.3.6 (R)-6-hydroxynicotine + H2O + electron acceptor as electron acceptors methylene blue and 2,6-dichlorophenolindophenol aerobically and anaerobically Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + electron donor
-
?
1.5.3.6 (R)-6-hydroxynicotine + H2O + O2
-
Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
?
1.5.3.6 additional information
-
Paenarthrobacter nicotinovorans ?
-
?

Subunits

EC Number Subunits Comment Organism
1.5.3.6 monomer
-
Paenarthrobacter nicotinovorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.3.6 45
-
inactivation above this temperature Paenarthrobacter nicotinovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.6 8
-
in 0.1 M Tris-HCl buffer Paenarthrobacter nicotinovorans
1.5.3.6 8.5
-
0.1 M glycine-NaOH buffer Paenarthrobacter nicotinovorans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.3.6 6
-
below this pH inactivation Paenarthrobacter nicotinovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.6 FAD one mol FAD per mol enzyme Paenarthrobacter nicotinovorans
1.5.3.6 FAD covalently bound to a histidine Paenarthrobacter nicotinovorans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.3.6 0.05
-
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one
-
Paenarthrobacter nicotinovorans
1.5.3.6 0.7
-
3-(4-aminobutyl)-pyridine
-
Paenarthrobacter nicotinovorans
1.5.3.6 1.5
-
6-hydroxy-L-nicotine
-
Paenarthrobacter nicotinovorans
1.5.3.6 1.7
-
D,L-2-Hydroxynicotine
-
Paenarthrobacter nicotinovorans