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Literature summary extracted from
- Nicotine dehydrogenase from Arthrobacter oxidans: A molybdenum-containing hydroxylase (1988), FEMS Microbiol. Lett., 52, 13-18.
No PubMed abstract available
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.99.4 | Fe2+ | four iron groups per mol | Pseudarthrobacter oxydans |  |
| 1.5.99.4 | Molybdenum | one molecule per mol | Pseudarthrobacter oxydans | |
| 1.5.99.4 | additional information | two labile sulfur groups per mol | Pseudarthrobacter oxydans | |
| 1.5.99.14 | Iron | contains 1 mol FAD, 1 mol molybdenum, 4 mol iron and 2 mol labile sulfur per mol of enzyme | Pseudarthrobacter oxydans | |
| 1.5.99.14 | Molybdenum | contains 1 mol FAD, 1 mol molybdenum, 4 mol iron and 2 mol labile sulfur per mol of enzyme | Pseudarthrobacter oxydans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.99.4 | 15100 | - |
1 * 82000 + 1 * 30000 + 1 * 15100, SDS-PAGE | Pseudarthrobacter oxydans |
| 1.5.99.4 | 30000 | - |
1 * 82000 + 1 * 30000 + 1 * 15100, SDS-PAGE | Pseudarthrobacter oxydans |
| 1.5.99.4 | 82000 | - |
1 * 82000 + 1 * 30000 + 1 * 15100, SDS-PAGE | Pseudarthrobacter oxydans |
| 1.5.99.4 | 120000 | - |
gel filtration | Pseudarthrobacter oxydans |
| 1.5.99.14 | 15000 | - |
1 * 82000, 1 * 30000, 1 * 15000, SDS-PAGE | Pseudarthrobacter oxydans |
| 1.5.99.14 | 30000 | - |
1 * 82000, 1 * 30000, 1 * 15000, SDS-PAGE | Pseudarthrobacter oxydans |
| 1.5.99.14 | 82000 | - |
1 * 82000, 1 * 30000, 1 * 15000, SDS-PAGE | Pseudarthrobacter oxydans |
| 1.5.99.14 | 120000 | - |
gel filtration | Pseudarthrobacter oxydans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.99.4 | nicotine + acceptor + H2O | Pseudarthrobacter oxydans | first enzyme in the nicotine degradation pathway | 6-hydroxynicotine + reduced acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.99.4 | Pseudarthrobacter oxydans | - |
P-34 DSM 419 | - |
| 1.5.99.14 | Pseudarthrobacter oxydans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.99.4 | ammonium sulfate precipitation, ion-exchange, gel filtration | Pseudarthrobacter oxydans |
| 1.5.99.4 | hydroxylapatite column | Pseudarthrobacter oxydans |
| 1.5.99.14 | - |
Pseudarthrobacter oxydans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.99.4 | 28.3 | - |
- |
Pseudarthrobacter oxydans |
| 1.5.99.14 | 28.3 | - |
37°C, pH 7.5 | Pseudarthrobacter oxydans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.99.4 | nicotine + acceptor + H2O | preferentially 2,6-dichlorophenolindophenol used as electron acceptor | Pseudarthrobacter oxydans | 6-hydroxynicotine + reduced acceptor | - |
? | |
| 1.5.99.4 | nicotine + acceptor + H2O | molybdate is required | Pseudarthrobacter oxydans | 6-hydroxynicotine + reduced acceptor | - |
? | |
| 1.5.99.4 | nicotine + acceptor + H2O | first enzyme in the nicotine degradation pathway | Pseudarthrobacter oxydans | 6-hydroxynicotine + reduced acceptor | - |
? | |
| 1.5.99.14 | 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + acceptor + H2O | - |
Pseudarthrobacter oxydans | 1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one + reduced acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.99.4 | trimer | 1 * 82000 + 1 * 30000 + 1 * 15100, SDS-PAGE | Pseudarthrobacter oxydans |
| 1.5.99.14 | heterotrimer | 1 * 82000, 1 * 30000, 1 * 15000, SDS-PAGE | Pseudarthrobacter oxydans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.99.4 | 37 | - |
assay at | Pseudarthrobacter oxydans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.5.99.4 | 7.5 | - |
assay at | Pseudarthrobacter oxydans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.99.4 | FAD | one mol per mol | Pseudarthrobacter oxydans | |
| 1.5.99.4 | FAD | not covalently bound | Pseudarthrobacter oxydans | |
| 1.5.99.4 | additional information | molybdo-iron-sulfur-flavoprotein | Pseudarthrobacter oxydans | |
| 1.5.99.4 | pterin | part of molybdenum cofactor | Pseudarthrobacter oxydans | |
| 1.5.99.14 | FAD | contains 1 mol FAD, 1 mol molybdenum, 4 mol iron and 2 mol labile sulfur per mol of enzyme | Pseudarthrobacter oxydans | |
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