BRENDA - Enzyme Database

Purification and characterization of a doxorubicin-inhibited NADH-quinone (NADH-ferricyanide) reductase from rat liver plasma membranes

Kim, C.; Crane, F.L.; Faulk, W.P.; Morre, D.J.; J. Biol. Chem. 277, 16441-16447 (2002)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
7.1.1.2
medicine
the doxorubicin-inhibited NADH-quinone-reductase may provide a target for the anthracycline antitumor agents
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
7.1.1.2
doxorubicin
; maximal inhibition at 0.001 mM
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.1.1.2
plasma membrane
-
Rattus norvegicus
5886
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7.1.1.2
57000
-
x * 57000, SDS-PAGE
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.1.1.2
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
7.1.1.2
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
7.1.1.2
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.1.1.2
0.186
-
NADH-ubiquinone-0 oxidoreductase activity
Rattus norvegicus
7.1.1.2
10.3
-
NADH-ferricyanide oxidoreductase activity; reduction of K3Fe(CN)6
Rattus norvegicus
7.1.1.2
13.2
-
reduction of Coenzyme Q10
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.1.1.2
NADH + coenzyme Q10
-
392693
Rattus norvegicus
?
-
-
-
?
7.1.1.2
NADH + Fe(CN)63-
-
392693
Rattus norvegicus
?
-
-
-
?
7.1.1.2
NADH + ubiquinone
-
392693
Rattus norvegicus
NAD+ + ubiquinol
-
-
-
?
7.1.1.2
NADH + ubiquinone-0
-
392693
Rattus norvegicus
NAD+ + ubiquinol-0
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
7.1.1.2
?
x * 57000, SDS-PAGE
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
7.1.1.2
NADH
-
Rattus norvegicus
Application (protein specific)
EC Number
Application
Commentary
Organism
7.1.1.2
medicine
the doxorubicin-inhibited NADH-quinone-reductase may provide a target for the anthracycline antitumor agents
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
7.1.1.2
NADH
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
7.1.1.2
doxorubicin
; maximal inhibition at 0.001 mM
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
7.1.1.2
plasma membrane
-
Rattus norvegicus
5886
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7.1.1.2
57000
-
x * 57000, SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
7.1.1.2
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
7.1.1.2
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.1.1.2
0.186
-
NADH-ubiquinone-0 oxidoreductase activity
Rattus norvegicus
7.1.1.2
10.3
-
NADH-ferricyanide oxidoreductase activity; reduction of K3Fe(CN)6
Rattus norvegicus
7.1.1.2
13.2
-
reduction of Coenzyme Q10
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.1.1.2
NADH + coenzyme Q10
-
392693
Rattus norvegicus
?
-
-
-
?
7.1.1.2
NADH + Fe(CN)63-
-
392693
Rattus norvegicus
?
-
-
-
?
7.1.1.2
NADH + ubiquinone
-
392693
Rattus norvegicus
NAD+ + ubiquinol
-
-
-
?
7.1.1.2
NADH + ubiquinone-0
-
392693
Rattus norvegicus
NAD+ + ubiquinol-0
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
7.1.1.2
?
x * 57000, SDS-PAGE
Rattus norvegicus