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Literature summary extracted from

  • Yano, T.; Yagi, T.; Sledๆ, V.D.; Ohnishi, T.
    Expression and characterization of the 66-kilodalton (NQO3) ison-sulfur subunit of the proton-translocating NADH-quinone oxidoreducrase of Paracoccus denitrificans (1995), J. Biol. Chem., 270, 18264-18270.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.2 NQO3 subunit overexpressed in NQO3 Paracoccus denitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.2 Iron the enzyme contains at least 5 EPR-visible iron-sulfur clusters. The NQO3 subunits contains at least two distinct iron-sulfur clusters: 1 [2Fe-2S] cluster with axial EPR signals and a [4Fe-4S] cluster with rhombic symmetry. The midpoint redox potentials of [2Fe-2S] and [4Fe-4S] clusters at pH 8.6 is -472 mV and -391 mV, respectively Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.2 NADH + ubiquinone Paracoccus denitrificans proton-translocating enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Paracoccus denitrificans
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
7.1.1.2 the tetranuclear cluster in the isolated NQO3 subunit is sensitive towards oxidants and converts into [3Fe-4S] form Paracoccus denitrificans

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.2 recombinant NQO3 subunit Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 NADH + ubiquinone proton-translocating enzyme Paracoccus denitrificans NAD+ + ubiquinol
-
 ?
7.1.1.2 NADH + ubiquinone proton-translocating enzyme Paracoccus denitrificans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.2 More proton-translocating NADH-quinone oxidoreductase is composed of at least 14 dissimilar subunits, designated NQO1-14. NQO1, NQO2, NQO3, NQO9, and probably NQO6 subunits are cofactor binding subunits Paracoccus denitrificans

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 NADH-quinone oxidoreductase
-
 Paracoccus denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.2 FMN enzyme contains one noncovalently bound FMN Paracoccus denitrificans
7.1.1.2 NADH
-
 Paracoccus denitrificans