Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hossain, M.A.; Asada, K.
    Monodehydroascorbate reductase from cucumber is a flavin adenine dinucleotide enzyme (1985), J. Biol. Chem., 260, 12920-12926.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.4 2-Iodoacetamide 25 mM, 21% inhibition Cucumis sativus
1.6.5.4 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Cucumis sativus
1.6.5.4 NEM 25 mM, 34% inhibition Cucumis sativus
1.6.5.4 PCMB 0.05 mM, 82% inhibition Cucumis sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.4 0.0046
-
 NADH
-
 Cucumis sativus
1.6.5.4 0.012
-
 ferricyanide
-
 Cucumis sativus
1.6.5.4 0.023
-
 NADPH
-
 Cucumis sativus
1.6.5.4 0.03
-
 2,6-dichloroindophenol
-
 Cucumis sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.4 47000
-
 gel filtration Cucumis sativus

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.4 Cucumis sativus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.4
-
 Cucumis sativus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.6.5.4 NADH + H+ + 2 monodehydroascorbate = NAD+ + 2 ascorbate ping pong mechanism Cucumis sativus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.4 fruit
-
 Cucumis sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.4 256
-
-
 Cucumis sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.4 ferricyanide + NADPH + H+
-
 Cucumis sativus ferrocyanide + NADP+
-
 r
1.6.5.4 NADH + 2,6-dichlorophenol indophenol
-
 Cucumis sativus NAD+ + ?
-
 ?
1.6.5.4 NADH + monodehydroascorbate
-
 Cucumis sativus NAD+ + ascorbate
-
 ?
1.6.5.4 NADPH + monodehydroascorbate
-
 Cucumis sativus NADP+ + ascorbate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.4 monomer 1 * 47000, SDS-PAGE Cucumis sativus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.5.4 150
-
 NADPH
-
 Cucumis sativus
1.6.5.4 200
-
 NADH
-
 Cucumis sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.5.4 7.9
-
-
 Cucumis sativus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.5.4 5.5 9.5 pH 5.5: about 60% of maximal activity, pH 9.5: about 75% of maximal activity Cucumis sativus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.4 NADH
-
 Cucumis sativus
1.6.5.4 NADPH
-
 Cucumis sativus