Literature summary extracted from

Campbell, W.H.; Smarrelli, J.
Purification and kinetics of higher plant NADH:nitrate reductase (1978), Plant Physiol., 61, 611-616.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.7.1.1	cyanide	-	Cucurbita pepo
1.7.1.1	cyanide	-	Zea mays
1.7.1.1	NAD+	competitive towards NADH	Cucurbita pepo
1.7.1.1	NAD+	competitive towards NADH	Zea mays
1.7.1.1	nitrite	competitive towards nitrate	Cucurbita pepo
1.7.1.1	nitrite	competitive towards nitrate	Zea mays
1.7.1.1	p-hydroxymercuribenzoate	-	Cucurbita pepo

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.7.1.1	0.003	-	NADH	-	Zea mays
1.7.1.1	0.07	-	nitrate	-	Zea mays

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.7.1.1	Cucurbita pepo	-	-	-
1.7.1.1	Zea mays	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.7.1.1	-	Zea mays
1.7.1.1	-	Cucurbita pepo

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
1.7.1.1	nitrite + NAD+ + H2O = nitrate + NADH + H+	two-site ping-pong mechanism	Cucurbita pepo	a

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.7.1.1	cotyledon	-	Cucurbita pepo	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.7.1.1	0.89	-	-	Zea mays
1.7.1.1	1.9	-	-	Cucurbita pepo
1.7.1.1	2	-	-	Cucurbita pepo

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.7.1.1	nitrate + NADH	-	Zea mays	nitrite + NAD+ + H2O	-	?
1.7.1.1	nitrate + NADH	-	Cucurbita pepo	nitrite + NAD+ + H2O	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.7.1.1	7.5	-	-	Zea mays
1.7.1.1	7.5	-	-	Cucurbita pepo

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.7.1.1	NADH	-	Zea mays
1.7.1.1	NADH	-	Cucurbita pepo

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.7.1.1	additional information	-	additional information	-	Zea mays
1.7.1.1	additional information	-	additional information	-	Cucurbita pepo

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Release 2023.1 (January 2023)