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Literature summary extracted from

  • Smarrelli, J.; Campbell, W.H.
    Activation of Thalassiosira pseudonana NADH: nitrate reductase (1980), Phytochemistry, 19, 1601-1605.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.1.1 2-mercaptoethylamine 5 mM, 5.6fold stimulation Thalassiosira pseudonana
1.7.1.1 GSH 5 mM, 3.8fold stimulation Thalassiosira pseudonana
1.7.1.1 L-Cys 5 mM, 5.6fold stimulation Thalassiosira pseudonana
1.7.1.1 L-cysteic acid 5 mM, 2fold stimulation Thalassiosira pseudonana
1.7.1.1 L-cysteine methyl ester 5 mM, 5.6fold stimulation Thalassiosira pseudonana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.1 azide sodium azide Thalassiosira pseudonana
1.7.1.1 p-hydroxymercuribenzoate
-
 Thalassiosira pseudonana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.1 0.008
-
 NADH
-
 Thalassiosira pseudonana
1.7.1.1 0.04
-
 nitrate
-
 Thalassiosira pseudonana

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Thalassiosira pseudonana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.1
-
 Thalassiosira pseudonana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.1 4
-
-
 Thalassiosira pseudonana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 nitrate + NADH
-
 Thalassiosira pseudonana nitrite + NAD+ + H2O
-
 ?
1.7.1.1 reduced methyl viologen + nitrate
-
 Thalassiosira pseudonana methyl viologen + nitrite
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.1 7.5
-
-
 Thalassiosira pseudonana

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.1 NADH
-
 Thalassiosira pseudonana