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Literature summary extracted from

  • Blasco, R.; Castillo, F.; Martinez-Luque, M.
    The assimilatory nitrate reductase from the phototrophic bacterium, Rhodobacter capsulatus E1F1, is a flavoprotein (1997), FEBS Lett., 414, 45-49.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.1 0.052
-
 NADH reaction with nitrate Rhodobacter capsulatus
1.7.1.1 0.067
-
 NADH reaction with 2,6-dichlorophenol indophenol Rhodobacter capsulatus
1.7.1.1 0.096
-
 nitrate reaction with NADH Rhodobacter capsulatus
1.7.1.1 0.13
-
 nitrate reaction with methyl viologen Rhodobacter capsulatus
1.7.1.1 0.54
-
 reduced bromophenol blue
-
 Rhodobacter capsulatus
1.7.1.1 0.87
-
 reduced methyl viologen
-
 Rhodobacter capsulatus
1.7.1.1 13
-
 nitrate reaction with bromophenol blue Rhodobacter capsulatus
1.7.1.1 80
-
 ferricytochrome c
-
 Rhodobacter capsulatus
1.7.1.1 100
-
 oxidized 2,6-dichlorophenolindophenol
-
 Rhodobacter capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.1 46000
-
 1 * 46000 + 1 * 95000, SDS-PAGE Rhodobacter capsulatus
1.7.1.1 95000
-
 1 * 46000 + 1 * 95000, SDS-PAGE Rhodobacter capsulatus
1.7.1.1 144000
-
 non-denaturing pore gradient PAGE Rhodobacter capsulatus
1.7.1.1 147000
-
 gel filtration Rhodobacter capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Rhodobacter capsulatus
-
 E1F1
-
1.7.1.1 Rhodobacter capsulatus E1F1
-
 E1F1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.1
-
 Rhodobacter capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.1 additional information
-
-
 Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 2 ferricytochrome c + NADH
-
 Rhodobacter capsulatus 2 ferrocytochrome c + NAD+ + H+
-
 ?
1.7.1.1 2 ferricytochrome c + NADH
-
 Rhodobacter capsulatus E1F1 2 ferrocytochrome c + NAD+ + H+
-
 ?
1.7.1.1 nitrate + NADH
-
 Rhodobacter capsulatus nitrite + NAD+ + H2O
-
 ?
1.7.1.1 nitrate + NADH
-
 Rhodobacter capsulatus E1F1 nitrite + NAD+ + H2O
-
 ?
1.7.1.1 oxidized 2,6-dichlorophenolindophenol + NADH
-
 Rhodobacter capsulatus ? + NAD+
-
 ?
1.7.1.1 oxidized 2,6-dichlorophenolindophenol + NADH
-
 Rhodobacter capsulatus E1F1 ? + NAD+
-
 ?
1.7.1.1 oxidized 2,6-dichlorophenolindophenol + NADH + H+
-
 Rhodobacter capsulatus reduced 2,6-dichlorophenolindophenol + NAD+
-
 r
1.7.1.1 reduced bromophenol blue + nitrate
-
 Rhodobacter capsulatus bromophenol blue + nitrite
-
 ?
1.7.1.1 reduced bromophenol blue + nitrate
-
 Rhodobacter capsulatus E1F1 bromophenol blue + nitrite
-
 ?
1.7.1.1 reduced methyl viologen + nitrate
-
 Rhodobacter capsulatus methyl viologen + nitrite
-
 ?
1.7.1.1 reduced methyl viologen + nitrate
-
 Rhodobacter capsulatus E1F1 methyl viologen + nitrite
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.1 dimer 1 * 46000 + 1 * 95000, SDS-PAGE Rhodobacter capsulatus
1.7.1.1 More diaphorase activity is located in the small subunit Rhodobacter capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.1 8.5
-
 NADH-diaphorase activity Rhodobacter capsulatus
1.7.1.1 9
-
 NADH-nitrate reductase activity Rhodobacter capsulatus
1.7.1.1 9.5
-
 methyl viologen-nitrate reductase activity Rhodobacter capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.1 NADH
-
 Rhodobacter capsulatus